Anticorps Polyclonal de lapin anti-PADI2

• Phare
• Validé par KD/KO

PADI2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, COIP, Blocking assay, ELISA

Conjugaison

Non conjugué

Publications

51

N° de cat : 12110-1-AP

Synonymes

KIAA0994, PAD H19, PAD2, PADI2, PDI2, Peptidylarginine deiminase II

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Galerie de données de validation

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  WB analysis using 12110-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 12110-1-AP

  WB result of PADI2 antibody (12110-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PADI2 transfected MCF-7 cells.

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  WB analysis of MCF-7 using 12110-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12110-1-AP (PADI2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12110-1-AP

  IP Result of anti-PADI2 (IP:12110-1-AP, 3ug; Detection:12110-1-AP 1:300) with mouse brain tissue lysate 3600ug.

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  IHC staining of human breast cancer using 12110-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12110-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12110-1-AP (PADI2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of BxPC-3 using 12110-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 12110-1-AP (PADI2 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 12110-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 12110-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 12110-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12110-1-AP

  Immunofluorescent analysis of HeLa cells using 12110-1-AP (PADI2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules BxPC-3, cellules HeLa, tissu de cancer du sein humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 12 publications below
• WB: See 35 publications below
• IHC: See 12 publications below
• IF: See 19 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12110-1-AP cible PADI2 dans les applications de WB, IP, IHC, IF, FC, COIP, Blocking assay, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PADI2 Protéine recombinante Ag2755
• Nom complet: peptidyl arginine deiminase, type II
• Masse moléculaire calculée: 665 aa, 75 kDa
• Poids moléculaire observé: 70-75 kDa, 50 kDa
• Numéro d’acquisition GenBank: BC009701
• Symbole du gène: PADI2
• Identification du gène (NCBI): 11240
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PADI2, also named as KIAA0994, PDI2, PAD-H19, and PAD2(Peptidylarginine deiminase II ), belongs to the protein arginine deiminase family. It catalyzes the deimination of arginine residues of proteins. PADI2 may play a regulatory role in the expression of lactation-related genes via histone citrullination during diestrus (PMID:20668670). PADI2 has two isoforms with MW 75 kDa and 49 kDa.

Publications

Species	Application	Title
human	WB,IF	Sci Transl Med; Neutrophil extracellular traps, B cells, and type I interferons contribute to immune dysregulation in hidradenitis suppurativa.; Authors - Angel S Byrd
human	WB	Mol Cell; Arginine Citrullination at the C-Terminal Domain Controls RNA Polymerase II Transcription.; Authors - Priyanka Sharma ; KD Validated
mouse	WB,FC	J Immunother Cancer; PAD-2-mediated citrullination of nucleophosmin provides an effective target for tumor immunotherapy.; Authors - Ruhul H Choudhury ; KO Validated
mouse	WB,IF	Angiogenesis; Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.; Authors - Jing Bai ; KD Validated
human	WB	Proc Natl Acad Sci U S A; Peptidylarginine deiminase 2-catalyzed histone H3 arginine 26 citrullination facilitates estrogen receptor α target gene activation.; Authors - Zhang Xuesen X
human	WB	Diabetes; Modifying Enzymes are Elicited by ER Stress, Generating Epitopes that are Selectively Recognized by CD4+ T Cells in Patients With Type 1 Diabetes.; Authors - Meghan L Marre

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