Anticorps Monoclonal anti-Serpin E1/PAI-1
Serpin E1/PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Hôte / Isotype
Mouse / IgG1
Réactivité testée
Humain, porc, souris et plus (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugaison
Non conjugué
CloneNo.
1H4A5
N° de cat : 66261-1-Ig
Synonymes
Galerie de données de validation
Applications testées
Résultats positifs en WB | plaquettes de sang périphérique humain, cellules HuH-7, cellules SH-SY5Y, cellules U-251, tissu hépatique de porc |
Résultats positifs en IHC | tissu rénal humain, tissu pulmonaire de souris il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Résultats positifs en IF/ICC | cellules HepG2, |
Dilution recommandée
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochimie (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Applications publiées
KD/KO | See 1 publications below |
WB | See 20 publications below |
IHC | See 4 publications below |
IF | See 7 publications below |
Informations sur le produit
66261-1-Ig cible Serpin E1/PAI-1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris
Réactivité | Humain, porc, souris |
Réactivité citée | rat, Humain, porc, souris |
Hôte / Isotype | Mouse / IgG1 |
Clonalité | Monoclonal |
Type | Anticorps |
Immunogène | Serpin E1/PAI-1 Protéine recombinante Ag22411 |
Nom complet | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
Masse moléculaire calculée | 402 aa, 45 kDa |
Poids moléculaire observé | 45-50 kDa |
Numéro d’acquisition GenBank | BC010860 |
Symbole du gène | PAI-1 |
Identification du gène (NCBI) | 5054 |
Conjugaison | Non conjugué |
Forme | Liquide |
Méthode de purification | Purification par protéine G |
Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.
Protocole
Product Specific Protocols | |
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WB protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
IHC protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
IF protocol for Serpin E1/PAI-1 antibody 66261-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Aging Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer | ||
Front Immunol Immunological characterization of stroke-heart syndrome and identification of inflammatory therapeutic targets | ||
Int J Mol Sci Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5. | ||
Am J Respir Cell Mol Biol ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1. | ||
Clin Sci (Lond) Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability. | ||
Phytomedicine Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Mona (Verified Customer) (10-17-2021) | Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).
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