Anticorps Monoclonal anti-Serpin E1/PAI-1

Serpin E1/PAI-1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(29)

CloneNo.

1H4A5

N° de cat : 66261-1-Ig

Synonymes

PAI-1, SERPINE1, SERPINE1, PAI-1, 1H4A5, PAI



Applications testées

Résultats positifs en WBplaquettes de sang périphérique humain, cellules HuH-7, cellules SH-SY5Y, cellules U-251, tissu hépatique de porc
Résultats positifs en IHCtissu rénal humain, tissu pulmonaire de souris
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules HepG2,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66261-1-Ig cible Serpin E1/PAI-1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, porc, souris

Réactivité Humain, porc, souris
Réactivité citéerat, Humain, porc, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Serpin E1/PAI-1 Protéine recombinante Ag22411
Nom complet serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
Masse moléculaire calculée 402 aa, 45 kDa
Poids moléculaire observé45-50 kDa
Numéro d’acquisition GenBankBC010860
Symbole du gène PAI-1
Identification du gène (NCBI) 5054
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

SERPINE1, also named Plasminogen activator inhibitor type 1 (PAI-1), is a member of the serine protease inhibitor (SERPIN) superfamily. PAI-1 is the principal inhibitor of tissue plasminogen activator (tPA) and uPA, and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. The mature 379-residue protein had a calculated unglycosylated molecular mass of 42.7 kD. Three putative glycosylation sites were found.

Protocole

Product Specific Protocols
WB protocol for Serpin E1/PAI-1 antibody 66261-1-IgDownload protocol
IHC protocol for Serpin E1/PAI-1 antibody 66261-1-IgDownload protocol
IF protocol for Serpin E1/PAI-1 antibody 66261-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Nat Aging

Single-cell and spatial RNA sequencing identify divergent microenvironments and progression signatures in early- versus late-onset prostate cancer

Authors - Yifei Cheng
mouseWB

Front Immunol

Immunological characterization of stroke-heart syndrome and identification of inflammatory therapeutic targets

Authors - Junyi Zheng
mouseWB

Int J Mol Sci

Identification of Impacted Pathways and Transcriptomic Markers as Potential Mediators of Pulmonary Fibrosis in Transgenic Mice Expressing Human IGFBP5.

Authors - Xinh-Xinh Nguyen
humanWB

Am J Respir Cell Mol Biol

ZMPSTE24 Regulates SARS-CoV-2 Spike Protein-enhanced Expression of Endothelial Plasminogen Activator Inhibitor-1.

Authors - Mingming Han
mouseWB

Clin Sci (Lond)

Anti-anemia drug FG4592 retards the AKI-to-CKD transition by improving vascular regeneration and antioxidative capability.

Authors - Mengqiu Wu
humanWB

Phytomedicine

Rhamnocitrin extracted from Nervilia fordii inhibited vascular endothelial activation via miR-185/STIM-1/SOCE/NFATc3.

Authors - Tong Lin

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mona (Verified Customer) (10-17-2021)

Paraffin-embedded hamster tissue from the lung. Antigen retrieval was performed with Tris-EDTA buffer (pH 9).

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: hamster (lung)
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