Anticorps Polyclonal de lapin anti-PAICS

• Phare
• Validé par KD/KO

PAICS Polyclonal Antibody for FC, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 12967-1-AP

Synonymes

ADE2, ADE2H1, AIR carboxylase, AIRC, PAICS, PAIS

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Galerie de données de validation

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  WB analysis using 12967-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12967-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12967-1-AP (PAICS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12967-1-AP (PAICS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12967-1-AP (PAICS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12967-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12967-1-AP (PAICS antibody) at dilution of 1:50 (under 40x lens).

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  FC experiment of HeLa using 12967-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PAICS antibody (12967-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat, cellules K-562, cellules Raji, cellules THP-1, tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

12967-1-AP cible PAICS dans les applications de WB, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAICS Protéine recombinante Ag3621
• Nom complet: phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazole succinocarboxamide synthetase
• Masse moléculaire calculée: 425 aa, 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC010273
• Symbole du gène: PAICS
• Identification du gène (NCBI): 10606
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAICS is also named as ADE2(Multifunctional protein), AIRC, PAIS. It is an important bifunctional enzyme in de novo purine biosynthesis in vertebrate with both 5-aminoimidazole ribonucleotide carboxylase (AIRc) and 4-(N-succinylcarboxamide)-5-aminoimidazole ribonucleotide synthetase (SAICARs) activities. It becomes an attractive target for rational anticancer drug design, since rapidly dividing cancer cells rely heavily on the purine de novo pathway for synthesis of adenine and guanine, whereas normal cells favor the salvage pathway(PMID:17224163).

Publications

Species	Application	Title
human	WB	Mol Cell; ERK2 Phosphorylates PFAS to Mediate Posttranslational Control of De Novo Purine Synthesis.; Authors - Eunus S Ali
mouse	IF,CoIP	iScience; Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex.; Authors - Seiya Yamada ; KD Validated
human	WB	Biol Res; Knockdown of PAICS inhibits malignant proliferation of human breast cancer cell lines.; Authors - Minjun Meng ; KD Validated
human	WB	J Clin Invest; Therapeutic targeting of metabolic vulnerabilities in cancers with MLL3/4-COMPASS epigenetic regulator mutations; Authors - Zibo Zhao
mouse	WB	eNeuro; Spatiotemporal Regulation of De Novo and Salvage Purine Synthesis during Brain Development; Authors - Tomoya Mizukoshi