Anticorps Polyclonal de lapin anti-PAIP1

• Phare
• Validé par KD/KO

PAIP1 Polyclonal Antibody for IF, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe

Applications

WB, IP, IHC, IF,ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 10675-1-AP

Synonymes

PABP interacting protein 1, PAIP 1, PAIP1

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Galerie de données de validation

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  WB analysis of HeLa using 10675-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COS-7 using 10675-1-AP

  COS-7 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 10675-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10675-1-AP (PAIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10675-1-AP

  IP result of anti-PAIP1 (IP:10675-1-AP, 4ug; Detection:10675-1-AP 1:300) with HeLa cells lysate 3000 ug.

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  IF Staining of MCF-7 using 10675-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PAIP1 antibody 10675-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules BxPC-3, cellules COS-7
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

10675-1-AP cible PAIP1 dans les applications de WB, IP, IHC, IF,ELISA et montre une réactivité avec des échantillons Humain, singe

• Réactivité: Humain, singe
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAIP1 Protéine recombinante Ag1070
• Nom complet: poly(A) binding protein interacting protein 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 60 kDa, 51 kDa, 40 kDa
• Numéro d’acquisition GenBank: BC005295
• Symbole du gène: PAIP1
• Identification du gène (NCBI): 10605
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

In initiation of translation in eukaryotes, binding of the small ribosomal subunit to mRNA requires recognition of the 5-prime cap structure by the cap-binding complex eIF4F. eIF4F consists of eIF4E, eIF4A, and eIF4G. Translation initiation is further regulated by the mRNA 3-prime poly(A) tail and the poly(A)-binding protein (PABC1). PAIP1 interacts with PABC1 and some eIF4 complexes [PMID:9548260]. It is a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs, and associates with EIF4A and PABPC1 may potentiate contacts between mRNA termini [PMID:11051545].

Publications

Species	Application	Title
human	WB	Biochem J; Biological Insights into the Expression of Translation Initiation Factors from Recombinant CHOK1SV Cell Lines and their Relationship to Enhanced Productivity.; Authors - Emma Jane Mead
human	WB,IHC,IF	Biol Reprod; Murine PAIP1 stimulates translation of spermiogenic mRNAs stored by YBX2 via its interaction with YBX2.; Authors - Yue He
human	WB,IHC	Hum Pathol; Paip1 predicts poor prognosis and promotes tumor progression through AKT/GSK-3β pathway in lung adenocarcinoma.; Authors - Yixuan Wang ; KD Validated
human	WB,IF	Exp Cell Res; Role of Paip1 on angiogenesis and invasion in pancreatic cancer.; Authors - Hongjian Guan ; KD Validated
human	WB,IHC	Cancer Res Treat; Paip1 Indicated Poor Prognosis in Cervical Cancer and Promoted Cervical Carcinogenesis.; Authors - Nan Li ; KD Validated