Anticorps Polyclonal de lapin anti-PAK4

• Phare
• Validé par KD/KO

PAK4 Polyclonal Antibody for FC, IF, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

16

Avis (1)

N° de cat : 14685-1-AP

Synonymes

KIAA1142, p21 activated kinase 4, PAK 4, PAK4

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Galerie de données de validation

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  WB analysis of HeLa using 14685-1-AP

  WB result of PAK4 antibody (14685-1-AP; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-PAK4 transfected HeLa cells.

• 

  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 14685-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14685-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 14685-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 14685-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 14685-1-AP (PAK4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14685-1-AP

  IP Result of anti-PAK4 (IP:14685-1-AP, 4ug; Detection:14685-1-AP 1:800) with mouse liver tissue lysate 4000ug.

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  IF Staining of HeLa using 14685-1-AP

  Immunofluorescent analysis of Hela cells, using PAK4 antibody 14685-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  FC experiment of HeLa using 14685-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PAK4 antibody (14685-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules COLO 320, cellules HeLa, cellules PC-3, tissu cutané de rat
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IF: cellules HeLa
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 14 publications below
• IHC: See 6 publications below
• IF: See 5 publications below

Informations sur le produit

14685-1-AP cible PAK4 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAK4 Protéine recombinante Ag5981
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 4
• Masse moléculaire calculée: 64 kDa
• Poids moléculaire observé: 64 kDa
• Numéro d’acquisition GenBank: BC025282
• Symbole du gène: PAK4
• Identification du gène (NCBI): 10298
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAK4(p21-activated kinase 4) is also named as KIAA1142. It belongs to the protein kinase superfamily.PAK4 regulates cell morphology, cytoskeletal organization, and cell proliferation and migration. It can also function as an antiapoptotic protein (PMID:14517283). It has 4 isoforms produced by alternative splicing. And it can be autophosphorylated on serine residues when activated by CDC42/p21 (PMID:11278822).

Publications

Species	Application	Title
human	WB,IF	Nat Commun; Proximity proteomics identifies PAK4 as a component of Afadin-Nectin junctions.; Authors - Yohendran Baskaran ; KD Validated
human	WB,IHC	Cancer Res; Targeting PAK4 Inhibits Ras-Mediated Signaling and Multiple Oncogenic Pathways in High-Risk Rhabdomyosarcoma.; Authors - Atreyi Dasgupta
human	IHC,WB	Mol Ther Nucleic Acids; ZEB1-Mediated Transcriptional Upregulation of circWWC3 Promotes Breast Cancer Progression through Activating Ras Signaling Pathway.; Authors - Lingjiao Meng
human	WB	Aging (Albany NY); PAK4 suppresses TNF-induced release of endothelial microparticles in HUVECs cells.; Authors - Shouqin Zhang ; KD Validated
mouse	WB	Commun Biol; RhoGDIα regulates spermatogenesis through Rac1/cofilin/F-actin signaling; Authors - Haixia Zhu
mouse	WB,IF	J Biol Chem; The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.; Authors - Liza J Schiavi-Ehrenhaus

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emily (Verified Customer) (07-18-2022)

Good strong signal by western blot, intend to use for IF soon

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Breast cancer cell