Anticorps Polyclonal de lapin anti-PAK6

• Phare
• Validé par KD/KO

PAK6 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 13539-1-AP

Synonymes

p21 activated kinase 6, PAK 6, PAK6

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Galerie de données de validation

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  WB analysis using 13539-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 13539-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13539-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13539-1-AP (PAK6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 13539-1-AP

  IP result of anti-PAK6 (IP:13539-1-AP, 4ug; Detection:13539-1-AP 1:300) with mouse testis tissue lysate 4000 ug.

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 13539-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 13539-1-AP (PAK6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HEK-293 using 13539-1-AP

  Immunofluorescent analysis of HEK-293 cells using 13539-1-AP (PAK6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules Raji, tissu testiculaire de rat
• Résultats positifs en IP: tissu testiculaire de souris,
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

13539-1-AP cible PAK6 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAK6 Protéine recombinante Ag4467
• Nom complet: p21 protein (Cdc42/Rac)-activated kinase 6
• Masse moléculaire calculée: 681 aa, 75 kDa
• Poids moléculaire observé: 70-75 kDa
• Numéro d’acquisition GenBank: BC035596
• Symbole du gène: PAK6
• Identification du gène (NCBI): 56924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAK6(p21-activated kinase 6) is a serine/threonine kinase belonging to the p21-activated kinase (PAK) family. .This protein plays a role in the regulation of gene transcription and may protect cells from apoptosis through phosphorylation of BAD. Northern blot analysis revealed that PAK6 is highly expressed in testis and prostate tissues (PMID:11278661). The immunogen of this antibody is the C-terminal of PAK6. This antibody has weak cross-reaction with other PAK members.

Publications

Species	Application	Title
human	WB,IHC	Oncol Lett; PAK6 promotes cervical cancer progression through activation of the Wnt/β-catenin signaling pathway.; Authors - Qin Yang ; KD Validated
rat	WB	J Pain Res; Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation.; Authors - Yuanyuan Wu
human	WB, IHC	Mol Med Rep; PMID: 26459798; Authors - Chunhui Liu
human	WB	BMC Nephrol; Urinary exosome proteins PAK6 and EGFR as noninvasive diagnostic biomarkers of diabetic nephropathy; Authors - Tao Li