Anticorps Polyclonal de lapin anti-PAN2

• Phare
• Validé par KD/KO

PAN2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 16427-1-AP

Synonymes

hPAN2, KIAA0710, PAN2, USP52

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16427-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16427-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16427-1-AP (PAN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16427-1-AP (PAN2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human spleen using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16427-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16427-1-AP (PAN2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 16427-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16427-1-AP (PAN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HepG2
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral humain, tissu hépatique humain, tissu placentaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Informations sur le produit

16427-1-AP cible PAN2 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAN2 Protéine recombinante Ag9670
• Nom complet: PAN2 poly(A) specific ribonuclease subunit homolog (S. cerevisiae)
• Masse moléculaire calculée: 1198 aa, 135 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC024043
• Symbole du gène: PAN2
• Identification du gène (NCBI): 9924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Nat Commun; USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.; Authors - Shangda Yang ; KD Validated
	WB	Nat Struct Mol Biol; An asymmetric PAN3 dimer recruits a single PAN2 exonuclease to mediate mRNA deadenylation and decay.; Authors - Stefanie Jonas
human	IF	Neurobiol Dis; Distinct Poly(A) nucleases have differential impact on sut-2 dependent tauopathy phenotypes.; Authors - Rebecca L Kow
mouse	WB	Biol Reprod; Mobilization of Dormant Cnot7 mRNA Promotes Deadenylation of Maternal Transcripts During Mouse Oocyte Maturation.; Authors - Jun Ma
human	WB, IP	PLoS One; Anti-PABPC1 Co-Immunoprecipitation for Examining the miRNAs Directly Targeting the 3'-UTR of EED mRNA.; Authors - Yi Hu
mouse	WB	Cells; Translation Efficiency and Degradation of ER-Associated mRNAs Modulated by ER-Anchored poly(A)-Specific Ribonuclease (PARN).; Authors - Tian-Li Duan