Anticorps Polyclonal de lapin anti-PANX1

• Phare
• Validé par KD/KO

PANX1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(18)

N° de cat : 12595-1-AP

Synonymes

pannexin 1, OZEMA7, OOMD7, MRS1, Innexin

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Galerie de données de validation

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  WB analysis using 12595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 12595-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 12595-1-AP (PANX1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules C2C12, cellules K-562, cellules MDA-MB-231, cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

12595-1-AP cible PANX1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PANX1 Protéine recombinante Ag3174
• Nom complet: pannexin 1
• Masse moléculaire calculée: 426 aa, 48 kDa
• Poids moléculaire observé: 45-50 kDa, 90 kDa
• Numéro d’acquisition GenBank: BC016931
• Symbole du gène: PANX1
• Identification du gène (NCBI): 24145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PANX1, also named as MRS1, belongs to the pannexin family. It plays a role as a Ca2+-leak channel to regulate ER Ca2+ homeostasis. It is a structural component of the gap junctions and the hemichannels. PANX1 induced the formation of intercellular channels with characteristics of gap junctions. PANX1 is 48-kDa protein which contains 4 transmembrane domains and cytoplasmic N and C termini. It is a mediator of find-me signal/nucleotide release from apoptotic cells. Western blot for Panx1 was also seen in two bands at 45~50 kDa and ~90 kDa. The band of ~90 kDa corresponding to the Panx1 dimer size. (PMID: 24590064, PMID: 19009624)

Publications

Species	Application	Title
human	IHC	Theranostics; Mitochondrial Protein UQCRC1 is Oncogenic and a Potential Therapeutic Target for Pancreatic Cancer.; Authors - Qing Wang
human	WB	J Lipid Res; Hyperactivation of SREBP induces Pannexin-1-dependent lytic cell death; Authors - Yanni Xiong ; KO Validated
human	WB	Sci Signal; Structures of human pannexin-1 in nanodiscs reveal gating mediated by dynamic movement of the N terminus and phospholipids.; Authors - Maki Kuzuya
human	WB	PLoS Pathog; Defining the proteolytic landscape during enterovirus infection.; Authors - Mohsan Saeed
rat	WB	Int J Mol Sci; Age-Dependent Activation of Pannexin1 Function Contributes to the Development of Epileptogenesis in Autosomal Dominant Sleep-related Hypermotor Epilepsy Model Rats; Authors - Kouji Fukuyama
human	IHC	Front Oncol; Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma.; Authors - Xiaojie Song

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