Anticorps Polyclonal de lapin anti-PARD3

• Phare
• Validé par KD/KO

PARD3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

24

Avis (1)

N° de cat : 11085-1-AP

Synonymes

ASIP, Baz, Bazooka, CTCL tumor antigen se2 5, PAR 3, PAR3, PAR3 alpha, PAR3A, PAR3alpha, PARD 3, PARD3, PARD3A, SE2 5L16, SE2 5LT1, SE2 5T2

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Galerie de données de validation

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  WB analysis using 11085-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11085-1-AP (PARD3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 11085-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11085-1-AP (PARD3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 11085-1-AP

  IP Result of anti-PARD3 (IP:11085-1-AP, 4ug; Detection:11085-1-AP 1:200) with MCF-7 cells lysate 2900ug.

• 

  IHC staining of human colon cancer using 11085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11085-1-AP (PARD3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11085-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11085-1-AP (PARD3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of MCF-7 using 11085-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using PARD3 antibody (11085-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 11085-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 11085-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 11085-1-AP (PARD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 11085-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PARD3 antibody (11085-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules MCF-7
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

11085-1-AP cible PARD3 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PARD3 Protéine recombinante Ag1565
• Nom complet: par-3 partitioning defective 3 homolog (C. elegans)
• Masse moléculaire calculée: 151 kDa
• Poids moléculaire observé: 180 kDa, 140-150 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC011711
• Symbole du gène: PARD3
• Identification du gène (NCBI): 56288
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PARD3 (also known as ASIP, Par3, or Bazooka) is one of PARD proteins which are essential for asymmetric cell division and polarized growth. PARD3 is involved in the establishment of cell polarity and in the asymmetric cytokinesis. It plays a role in tight junctions at epithelial cell-cell contacts. PARD3 has three splice isoforms at 100 kDa, 150 kDa, and 180 kDa. This polyclonal antibody raised against C-terminal 281 amino acids of human PARD3 recognizes these three isoforms.

Publications

Species	Application	Title
mouse	IF	Nat Commun; EMX2-GPR156-Gαi reverses hair cell orientation in mechanosensory epithelia.; Authors - Katie S Kindt
mouse	IF	Matrix Biol; Ameloblastin promotes polarization of ameloblast cell lines in a 3-D cell culture system; Authors - Gayathri Visakan
chicken	IHC,IF	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin ; KD Validated
		FASEB J; Perturbation of epithelial apicobasal polarity by rhomboid family-1 gene overexpression.; Authors - Xue-Mei Peng
mouse	WB	iScience; Glucocorticoid receptor-mediated Nr1d1 chromatin circadian misalignment in stress-induced irritable bowel syndrome; Authors - Gen Zheng
mouse	IP	Front Cell Dev Biol; Nox1-based NADPH oxidase regulates the Par protein complex activity to control cell polarization; Authors - Alejandra Valdivia

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sarah (Verified Customer) (06-26-2019)

Western blot: Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-PARD-3 in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.Immunofluorescence: Cells fixed in MeOH at -20 degrees were stained with 1:200 primary antibody. After washing, coverslips were incubated in 1:500 AF488 secondary antibody. Following mounting, images were acquired using a confocal microscope. Staining was very weak, as is seen by the image (Green = PARD3, Blue = Hoescht)

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000 (WB), 1:200 (IF)
• Cell Tissue Type: MCF10A

PARD3 Antibody Western Blot,Immunofluorescence, validation (1:1000 (WB), 1:200 (IF) dilution) in MCF10A (Cat no:11085-1-AP)

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