Anticorps Polyclonal de lapin anti-PAWR

PAWR Polyclonal Antibody for FC, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC,ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 20688-1-AP

Synonymes

Par 4, PAR4, PAWR, Prostate apoptosis response 4 protein

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Galerie de données de validation

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  WB analysis of mouse heart using 20688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 20688-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 20688-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 20688-1-AP (PAWR Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  IHC staining of human breast cancer using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

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  IHC staining of human kidney using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 20688-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 20688-1-AP (PAWR Antibody) at dilution of 1:50.

• 

  FC experiment of HeLa using 20688-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human PAWR (20688-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

20688-1-AP cible PAWR dans les applications de WB, IHC, FC,ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PRKC, apoptosis, WT1, regulator
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: NM_002583
• Symbole du gène: PAWR
• Identification du gène (NCBI): 5074
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAWR, also named as PAR4, is a pro-apoptopic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. It induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. PAWR inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. It down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. PAWR may be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1. The antibody is specific to PAWR.

Publications

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	IHC	Cancer Cell Int; Expression of PAWR predicts prognosis of ovarian cancer.; Authors - Jiahong Tan