Anticorps Polyclonal de lapin anti-PAX2

PAX2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 29307-1-AP

Synonymes

paired box 2, Paired box protein Pax 2, PAX2

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Galerie de données de validation

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  WB analysis using 29307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29307-1-AP (PAX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de souris, tissu de cervelet de rat
• Résultats positifs en IHC: tissu rénal de souris, tissu de carcinome à cellules rénales humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below
• FC: See 1 publications below

Informations sur le produit

29307-1-AP cible PAX2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PAX2 Protéine recombinante Ag30717
• Nom complet: paired box 2
• Masse moléculaire calculée: 431 aa, 46 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC148710
• Symbole du gène: PAX2
• Identification du gène (NCBI): 5076
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX2 is a transcription factor, primarily expressed during embryonic development. It has a critical role in the development of the urogenital tract, the eyes, and the CNS. It plays a key role during renal development and to act as an oncogene favoring renal tumor growth. PAX2 was suggested as a sensitive and highly specific marker for renal cell carcinoma (RCC).

Publications

Species	Application	Title
human	IHC	Sci Bull (Beijing); Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.; Authors - Xiangmin Lv
human	FC	Cells; Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies.; Authors - Bella Rossbach
human	WB	J Cell Mol Med; Chemerin's Role in Endometrial Dysfunction: Insights From Transcriptomic Analysis; Authors - Ming Yu

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