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Anticorps Polyclonal de lapin anti-PAX8

PAX8 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB,ELISA

Conjugaison

Non conjugué

N° de cat : 22255-1-AP

Synonymes

paired box 8, Paired box protein Pax 8, PAX8

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of A2780 cells using PAX8 Polyclonal antibody (22255-1-AP)

    WB analysis of A2780 using 22255-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 22255-1-AP (PAX8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A2780 cells using PAX8 Polyclonal antibody (22255-1-AP)

    WB analysis of A2780 using 22255-1-AP

    A2780 cells were subjected to SDS PAGE followed by western blot with 22255-1-AP (PAX8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:10 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:10 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:10 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:25 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:25 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:10 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human kidney using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human kidney using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PAX8 Polyclonal antibody (22255-1-AP)

    IHC staining of human ovary tumor using 22255-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 22255-1-AP (PAX8 antibody) at dilution of 1:50 (under 40x lens).

Applications testées

Dilution recommandée

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

22255-1-AP cible PAX8 dans les applications de WB,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PAX8 Protéine recombinante Ag17759
Nom complet paired box 8
Masse moléculaire calculée 450 aa, 48 kDa
Numéro d’acquisition GenBankBC001060
Symbole du gène PAX8
Identification du gène (NCBI) 7849
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PAX8 is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain and a paired-type homeodomain. It is expressed during organogenesis of the thyroid gland, kidney and Mullerian system. It is thought to regulate the expression of Wilms tumor suppressor (WT1) gene and mutations in PAX8 have been associated with Wilms tumor cells, thyroid and ovarian carcinomas. PAX8 is a useful marker in distinguishing ovarian carcinomas from mammary carcinomas (PMID: 18724243). PAX8 is expressed in a high percentage of ovarian serous, endometrioid, and clear cell carcinomas, but only rarely in primary ovarian mucinous adenocarcinomas.

PAX8 has 5 isoforms with calculated MW 31kd, 34kd, 41kd, 43kd and 48kd. And there is a 60kd band corresponding to the electrophoretic mobility of PAX8 (PMID:15650356). Proteintech’s 10336-1-AP antibody has cross-reactivities with the other PAX family members.