Anticorps Polyclonal de lapin anti-PCK1/2

• Phare
• Validé par KD/KO

PCK1/2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(90)

Avis (1)

N° de cat : 16754-1-AP

Synonymes

PCK1, Serine-protein kinase PCK1, PEPCK-C, PEPCK1, EC:4.1.1.32

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Galerie de données de validation

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  WB analysis using 16754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1/2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16754-1-AP

  IP result of anti-PCK1/2 (IP:16754-1-AP, 4ug; Detection:16754-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 16754-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCK1/2 antibody (16754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu hépatique de rat
• Résultats positifs en IP: cellules HepG2
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 78 publications below
• IHC: See 8 publications below
• IF: See 15 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

16754-1-AP cible PCK1/2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCK1/2 Protéine recombinante Ag10261
• Nom complet: phosphoenolpyruvate carboxykinase 1 (soluble)
• Masse moléculaire calculée: 622 aa, 69 kDa
• Poids moléculaire observé: 66-69 kDa
• Numéro d’acquisition GenBank: BC023978
• Symbole du gène: PCK1
• Identification du gène (NCBI): 5105
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). This antibody has weak cross-reactivity with PCK2 protein due to high sequence homology between PCK1 and PCK2.

Publications

Species	Application	Title
human	WB,IP	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
mouse	WB	Cell Metab; Amino acid is a major carbon source for hepatic lipogenesis; Authors - Yilie Liao
mouse	WB	Nat Commun; Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.; Authors - Jingwei Ma
mouse	IF	Adv Healthc Mater; Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment.; Authors - Jingjing Gan
human	WB	Biomaterials; Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.; Authors - Meiling Wu
mouse	WB	Proc Natl Acad Sci U S A; Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.; Authors - Daniel Mauvoisin ; KO Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-18-2022)

Detection of PCK1 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

PCK1/2 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:16754-1-AP)

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