Anticorps Polyclonal de lapin anti-PCSK9

• Phare
• Validé par KD/KO

PCSK9 Polyclonal Antibody for FC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 27882-1-AP

Synonymes

FH3, HCHOLA3, LDLCQ1, NARC 1, NARC1, PC9, PCSK9, Proprotein convertase 9

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Galerie de données de validation

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  WB analysis using 27882-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27882-1-AP (PCSK9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27882-1-AP

  WB result of PCSK9 antibody (27882-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCSK9 transfected HepG2 cells.

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  FC experiment of HepG2 using 27882-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PCSK9 (27882-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HepG2, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

27882-1-AP cible PCSK9 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCSK9 Protéine recombinante Ag27254
• Nom complet: proprotein convertase subtilisin/kexin type 9
• Masse moléculaire calculée: 74 kDa
• Poids moléculaire observé: 72-78 kDa, 62 kDa
• Numéro d’acquisition GenBank: NM_174936.3
• Symbole du gène: PCSK9
• Identification du gène (NCBI): 255738
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels. (PMID: 30522786, PMID: 22493497)

Publications

Species	Application	Title
rat	IHC	Biochimie; High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats; Authors - Ya-Nan Ou-Yang
mouse	WB	bioRxiv; Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice; Authors - Zhiqiang Li
human,mouse	WB,IHC,IF	iScience; Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer; Authors - Qi-Chao Yang ; KD Validated
human,mouse	WB	FASEB J; Exogenous hydrogen sulfide alleviates hepatic endoplasmic reticulum stress via SIRT1/FoxO1/PCSK9 pathway in NAFLD; Authors - Xiaomeng Cui
human	WB,IF	JACC Basic Transl Sci; PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia; Authors - Meixin Zhang
human	WB,IHC	Oncol Lett; PCSK9 promotes tumor cell proliferation and migration by facilitating CCL25 secretion in esophageal squamous cell carcinoma; Authors - Haijun Wang