Anticorps Polyclonal de lapin anti-PCYT1B/PCYT1A

PCYT1B/PCYT1A Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 13765-1-AP

Synonymes

PCYT1B, CCT B, CCT beta, CCTB, CCT-beta

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Galerie de données de validation

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  WB analysis using 13765-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13765-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 13765-1-AP

  IP result of anti-PCYT1B (IP:13765-1-AP, 4ug; Detection:13765-1-AP 1:500) with human placenta tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: cellules LNCaP, tissu cérébral humain
• Résultats positifs en IP: tissu placentaire humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

13765-1-AP cible PCYT1B/PCYT1A dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PCYT1B/PCYT1A Protéine recombinante Ag4748
• Nom complet: phosphate cytidylyltransferase 1, choline, beta
• Masse moléculaire calculée: 351 aa, 40 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC045634
• Symbole du gène: PCYT1B
• Identification du gène (NCBI): 9468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PCYT1B (Phosphate Cytidylyltransferase 1B, Choline), also named as CTβ, CCTB and CCT-Beta, is encoded by the gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. PCYT1B encodes three isoforms of CTβ (CTβ1, CTβ2 and CTβ3). CTβ1, CTβ2 isoforms are present in humans and CTβ2 and CTβ3 isoforms have been identified in mice. The CTβ2 can be detected as 41 kDa, and CTβ3 can be detected as 39 kDa. This antibody can recognizes both PCYT1A and PCYT1B. PCYT1A usually exists in the form of a 80 kDa dimer.

Publications

Species	Application	Title
mouse	WB	Neurobiol Dis; Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis; Authors - Fan Zhang

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