Anticorps Polyclonal de lapin anti-PDCD6/ALG2

• Phare
• Validé par KD/KO

PDCD6/ALG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(23)

Avis (1)

N° de cat : 12303-1-AP

Synonymes

ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog

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Galerie de données de validation

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  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 12303-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 12303-1-AP

  IP result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded normal stomach slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 12303-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDCD6/ALG2 antibody (12303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of MDCK using 12303-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using PDCD6/ALG2 antibody (12303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules Jurkat, cellules K-562, cellules NIH/3T3, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer du foie humain, tissu d'estomac humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MDCK,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 18 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Informations sur le produit

12303-1-AP cible PDCD6/ALG2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDCD6/ALG2 Protéine recombinante Ag2949
• Nom complet: programmed cell death 6
• Masse moléculaire calculée: 191 aa, 22 kDa
• Poids moléculaire observé: 15-22 kDa
• Numéro d’acquisition GenBank: BC012384
• Symbole du gène: PDCD6
• Identification du gène (NCBI): 10016
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Programmed cell death protein 6 (PDCD6) is also named as ALG2. PDCD6 is an evolutionarily conserved Ca2+-binding protein. PDCD6 is required for coat protein complex II-dependent endoplasmic reticulum-to-Golgi apparatus vesicular transport in the cytoplasm (PMID: 39934130). PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. The nuclear PDCD6 regulates apoptosis and alternative splicing. And cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex-dependent membrane budding. (PMID: 38436472). PDCD6 also acts as a regulator of cytosolic DNA-induced immune responses by modulating STING transport (PMID: 34787301).

Publications

Species	Application	Title
human	WB	Nature; Structural basis for dimerization quality control.; Authors - Elijah L Mena
human	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	WB,IF	Cell; Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.; Authors - Colleen A McGourty ; KD Validated
human	WB	Mol Cell; Co-adaptor driven assembly of a CUL3 E3 ligase complex.; Authors - David Akopian
human	WB,IF	Cell Rep; Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.; Authors - Johannes Westman ; KD Validated
human	IF	J Cell Biol; Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters; Authors - Femmy C Stempels

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kristal (Verified Customer) (06-09-2025)

The PDCD6/ALG2 staining was not reaching all the cells. I am trying to use highly polarized MDCK cells. This specific antibody might not work well in MDCK cells. I fixed in 4% PFA and incubated in primary overnight with 0.1% Triton-X and 5% BSA.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: MDCK cells

PDCD6/ALG2 Antibody Immunofluorescence validation (1:200 dilution) in MDCK cells (Cat no:12303-1-AP)

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