Anticorps Polyclonal de lapin anti-PDE4C

PDE4C Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 21754-1-AP

Synonymes

DPDE1, PDE21, PDE4C

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Galerie de données de validation

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  WB analysis of HEK-293 using 21754-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21754-1-AP (PDE4C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 21754-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21754-1-AP (PDE4C antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

21754-1-AP cible PDE4C dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDE4C Protéine recombinante Ag16130
• Nom complet: phosphodiesterase 4C, cAMP-specific (phosphodiesterase E1 dunce homolog, Drosophila)
• Masse moléculaire calculée: 712 aa, 80 kDa
• Poids moléculaire observé: ~70 kDa
• Numéro d’acquisition GenBank: BC109067
• Symbole du gène: PDE4C
• Identification du gène (NCBI): 5143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE4C(cAMP-specific 3,5-cyclic phosphodiesterase 4C) is also named as DPDE1 and belongs to the PDE4 subfamily, which play a key role in lung inflammation and hyperresponsiveness. PDE4C promotes the hydrolysis of cAMP, and PKD2, functioning as a Ca2+ entry channel, may mediate local accumulation of Ca2+ that inhibits the activity of Ca2+-sensitive ADCY5(PMID:21670265). The three isoforms(84,75, and 64 kDa) of PDE4C have been detected in cytosolic, microsomal, and nuclear (PMID:21784969).

Publications

Species	Application	Title
human	WB	JCI Insight; Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth; Authors - Mi-Hyeon Jeong
mouse	WB	Mol Metab; Inhibition of prolyl hydroxylases increases hepatic insulin and decreases glucagon sensitivity by an HIF-2α-dependent mechanism.; Authors - Matthew Riopel
mouse	WB	Biochem Pharmacol; DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.; Authors - Heng Li
mouse	WB	Am J Physiol Renal Physiol; Inhibition of PDE4/PDE4B improves renal function and ameliorates inflammation in cisplatin-induced acute kidney injury.; Authors - Man Xu
human	WB	Atherosclerosis; A novel protein encoded by circLARP1B promotes the proliferation and migration of vascular smooth muscle cells by suppressing cAMP signaling; Authors - Peng Lu
	WB	J Cell Mol Med; Analysis of the effects of M2 macrophage-derived PDE4C on the prognosis, metastasis and immunotherapy benefit of osteosarcoma; Authors - Feng Pan

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