Anticorps Polyclonal de lapin anti-PDE4D

• Phare
• Validé par KD/KO

PDE4D Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 12918-1-AP

Synonymes

DPDE3, HSPDE4D, PDE43, PDE4D, PDE4DN2, STRK1

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Galerie de données de validation

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  WB analysis using 12918-1-AP

  K562 cell lysate were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12918-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 12918-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 12918-1-AP (PDE4D antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse colon using 12918-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 12918-1-AP (PDE4D Antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 12918-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 12918-1-AP (PDE4D antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 12918-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PDE4D antibody (12918-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu cérébral de souris, tissu pancréatique de souris
• Résultats positifs en IHC: tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:100-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 19 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

12918-1-AP cible PDE4D dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, Lapin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDE4D Protéine recombinante Ag3588
• Nom complet: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Masse moléculaire calculée: 809 aa, 91 kDa
• Poids moléculaire observé: 91 kDa
• Numéro d’acquisition GenBank: BC036319
• Symbole du gène: PDE4D
• Identification du gène (NCBI): 5144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer disease(PMID:17353396).

Publications

Species	Application	Title
mouse	WB	Cell Metab; FGF1 and insulin control lipolysis by convergent pathways.; Authors - Gencer Sancar
human	WB	Acta Pharm Sin B; Targeting PDE4 as a promising therapeutic strategy in chronic ulcerative colitis through modulating mucosal homeostasis.; Authors - Heng Li
mouse	WB	Cell Rep; miR-199a Links MeCP2 with mTOR Signaling and Its Dysregulation Leads to Rett Syndrome Phenotypes.; Authors - Keita Tsujimura
human	WB	JCI Insight; Inhibition of phosphodiesterase 4D suppresses mTORC1 signaling and pancreatic cancer growth; Authors - Mi-Hyeon Jeong
	WB	Diabetes; Salt inducible kinase 1 terminates cAMP signaling by an evolutionarily conserved negative feedback loop in beta cells.; Authors - Min-Jung Kim
human	WB,IHC,IF	Cancer Lett; PDE4D binds and interacts with YAP to cooperatively promote HCC progression.; Authors - Huili Ren ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

RICHA (Verified Customer) (10-09-2019)

Great and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from fibroblasts

PDE4D Antibody Western Blot, validation (1:500 dilution) in Protein from fibroblasts (Cat no:12918-1-AP)