Anticorps Polyclonal de lapin anti-PDE6A

PDE6A Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 21200-1-AP

Synonymes

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

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Galerie de données de validation

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  WB analysis using 21200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye tissue using 21200-1-AP

  WB results of PDE6A antibody (21200-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

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  WB analysis of human brain using 21200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of retinal tissue using 21200-1-AP

  IF stain of PDE6a antibody with retinal tissue by a customer. (PDE6a is in red and nuclei are blue).

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Applications testées

• Résultats positifs en WB: tissu oculaire de souris, tissu cérébral humain, tissu oculaire de rat
• Résultats positifs en IHC: tissu oculaire de souris, tissu de cancer du côlon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu rétinien

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

21200-1-AP cible PDE6A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDE6A Protéine recombinante Ag15562
• Nom complet: phosphodiesterase 6A, cGMP-specific, rod, alpha
• Masse moléculaire calculée: 860 aa, 100 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC035909
• Symbole du gène: PDE6A
• Identification du gène (NCBI): 5145
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
rat	WB	Toxicol Appl Pharmacol; Ocular toxicity of AUY922 in pigmented and albino rats.; Authors - Danielle Roman
mouse	WB	Dev Biol; Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Mouse models of human ocular disease for translational research.; Authors - Mark P Krebs