Anticorps Polyclonal de lapin anti-PDGFC

PDGFC Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 55076-1-AP

Synonymes

FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E

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Galerie de données de validation

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  WB analysis of MDA-MB-453s using 55076-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s
• Résultats positifs en IHC: tissu rénal humain, tissu de cancer de la thyroïde humain, tissu de côlon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

55076-1-AP cible PDGFC dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: platelet derived growth factor C
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: NM_016205
• Symbole du gène: PDGFC
• Identification du gène (NCBI): 56034
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: NaHCO3 0,1 M, glycine 0,1 M, azoture de sodium à 0,02 % et glycérol à 50 %, pH 7,3.
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDGFC is a member of the platelet-derived growth factor family. It is a monomeric protein secreted as a latent dimeric factor. Expression of PDGFC was highest in prostate, testis, and uterus, with weaker expression in spleen, thymus, and small intestine, and little expression in colon and peripheral blood leukocytes.

Publications

Species	Application	Title
human	WB	Br J Cancer; Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis.; Authors - Xiang Li
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou