Anticorps Polyclonal de lapin anti-PDH E1 Alpha

• Phare
• Validé par KD/KO

PDH E1 Alpha Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(85)

Avis (3)

N° de cat : 18068-1-AP

Synonymes

PDH E1α, PDHA1, EC:1.2.4.1, PDHA, PDHA 1

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Galerie de données de validation

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  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK293 cells using 18068-1-AP

  WB result of PDHA1 antibody (18068-1-AP, 1:1000) with si-control and si-PDHA1 transfected HEK293 cells.

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  WB analysis using 18068-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18068-1-AP (PDH E1α antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 18068-1-AP

  IP result of anti-PDH E1 Alpha (IP:18068-1-AP, 4ug; Detection:18068-1-AP 1:1000) with mouse kidney tissue lysate 4400ug.

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  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 18068-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18068-1-AP (PDH E1 Alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 18068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDH E1 Alpha antibody (18068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK293, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de rat
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 70 publications below
• IHC: See 5 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Informations sur le produit

18068-1-AP cible PDH E1 Alpha dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDH E1 Alpha Protéine recombinante Ag12701
• Nom complet: pyruvate dehydrogenase (lipoamide) alpha 1
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 39-48 kDa
• Numéro d’acquisition GenBank: BC002406
• Symbole du gène: PDHA1
• Identification du gène (NCBI): 5160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing with the molecular mass of 39-48 kDa. Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).

Publications

Species	Application	Title
mouse	WB,IP	Cell Res; Hypoxia induces mitochondrial protein lactylation to limit oxidative phosphorylation; Authors - Yunzi Mao
human	WB	Cell Res; AMPK targets PDZD8 to trigger carbon source shift from glucose to glutamine; Authors - Mengqi Li
mouse	WB	Cell Metab; Acetate enables metabolic fitness and cognitive performance during sleep disruption; Authors - Qinqin He
mouse	WB	Nat Cell Biol; RIP3 targets pyruvate dehydrogenase complex to increase aerobic respiration in TNF-induced necroptosis; Authors - ; KO Validated
rat	WB	Cell Stem Cell; A small-molecule cocktail promotes mammalian cardiomyocyte proliferation and heart regeneration.; Authors - Jianyong Du
human	IF	Cell Stem Cell; Mechano-oncogenic cytoskeletal remodeling drives leukemic transformation with mitochondrial vesicle-mediated STING activation; Authors - Zemin Song

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

kes (Verified Customer) (01-17-2022)

I used it for WB(1:1000) and got nice bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Liver cells

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Human Liver cells (Cat no:18068-1-AP)

FH

David (Verified Customer) (09-03-2019)

Good detection with 30µg of protein and dilution at 1/1000

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Brain

PDH E1 Alpha Antibody Western Blot, validation (1/1000 dilution) in Brain (Cat no:18068-1-AP)

FH

Svitlana (Verified Customer) (11-02-2017)

Loading:per well 15 ug/ulGradient gel 4-12%Transfer:Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking:SEA Block Blocking Buffer 1hImaging:Odyssey CLx Imaging

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line).

PDH E1 Alpha Antibody Western Blot validation (1:1000 dilution) in Whole tissue homogenate (human, mouse), mitochondria (human, mouse), whole cell homogenate (rat cell line), mitochondria (rat cell line). (Cat no:18068-1-AP)

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