Anticorps Polyclonal de lapin anti-ERp72

• Phare
• Validé par KD/KO

ERp72 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

37

N° de cat : 14712-1-AP

Synonymes

Cell and organelle markers, endoplasmic reticulum marker, ER marker, ER protein 70, ER protein 72, ERp 72, ERP70, ERP72, PDIA4, Protein disulfide isomerase A4

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Galerie de données de validation

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  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14712-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14712-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14712-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14712-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14712-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14712-1-AP

  IP Result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 14712-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of PC-3 using 14712-1-AP

  Immunofluorescent analysis of PC-3 cells, using PDIA4 antibody 14712-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green).

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  FC experiment of HepG2 using 14712-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HeLa, cellules HepG2, cellules L02, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules PC-3
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 26 publications below
• IHC: See 2 publications below
• IF: See 11 publications below
• CoIP: See 1 publications below

Informations sur le produit

14712-1-AP cible ERp72 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: ERp72 Protéine recombinante Ag6431
• Nom complet: protein disulfide isomerase family A, member 4
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 72 kDa
• Numéro d’acquisition GenBank: BC000425
• Symbole du gène: PDIA4
• Identification du gène (NCBI): 9601
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Publications

Species	Application	Title
mouse	WB	Cell; MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.; Authors - Xiaorong Zhang
human	WB	J Thromb Haemost; SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia; Authors - Jiasheng Zhang
		Elife; Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.; Authors - Lars Plate
human	WB	Cell Chem Biol; Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.; Authors - Lars Plate
mouse	WB	Elife; PDIA1/P4HB is required for efficient proinsulin maturation and ß cell health in response to diet induced obesity.; Authors - Insook Jang
human	WB,CoIP	Mol Cell Proteomics; Thyroglobulin Interactome Profiling Defines Altered Proteostasis Topology Associated With Thyroid Dyshormonogenesis; Authors - Madison T. Wright