Anticorps Polyclonal de lapin anti-PDLIM1/CLP36

• Phare
• Validé par KD/KO

PDLIM1/CLP36 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 11674-1-AP

Synonymes

CLIM1, CLP 36, CLP36, Elfin, hCLIM1, LIM domain protein CLP 36, PDLIM1, PDLIM1/CLP36, PDZ and LIM domain 1, PDZ and LIM domain protein 1

---

Galerie de données de validation

• 

  WB analysis using 11674-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 11674-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human ileum using 11674-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11674-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11674-1-AP (PDLIM1/CLP36 antibody at dilution of 1:600 incubated at 4 degree celsius over night.

• 

  IP experiment of mouse heart using 11674-1-AP

  IP Result of anti-PDLIM1/CLP36 (IP:11674-1-AP, 3ug; Detection:11674-1-AP 1:600) with mouse heart tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 11674-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11674-1-AP (PDLIM1/CLP36 antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PDLIM1 antibody 11674-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  IF Staining of MCF-7 using 11674-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDLIM1/CLP36 antibody (11674-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of MCF-7 using 11674-1-AP

  1X10^6 MCF-7 cells were stained with 0.2ug PDLIM1/CLP36 antibody (11674-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: cellules C2C12, poumon de souris, tissu cardiaque de souris, tissu d'iléon humain, tissu pulmonaire humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 3 publications below

Informations sur le produit

11674-1-AP cible PDLIM1/CLP36 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDLIM1/CLP36 Protéine recombinante Ag2274
• Nom complet: PDZ and LIM domain 1
• Masse moléculaire calculée: 329 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC000915
• Symbole du gène: PDLIM1
• Identification du gène (NCBI): 9124
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Publications

Species	Application	Title
mouse	WB,IF	Autophagy; Autophagy is required for ectoplasmic specialization assembly in Sertoli cells.; Authors - Chao Liu ; KD Validated
mouse	WB	Autophagy; Autophagy regulates spermatid differentiation via degradation of PDLIM1.; Authors - Yongliang Shang
human	WB	EMBO Rep; Acetyltransferase GCN5 regulates autophagy and lysosome biogenesis by targeting TFEB.; Authors - Yusha Wang
mouse	WB	Open Biol; Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36.; Authors - Shiguo Li
human	WB,IF	Exp Cell Res; CLP36 and RIL recruit α-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts.; Authors - Miyazaki Kazufumi K ; KD Validated
human,rat	WB,IF	Neurosci Lett; CLP36 interacts with palladin in dorsal root ganglion neurons.; Authors - Hasegawa Tomohiko T

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Joleen (Verified Customer) (06-03-2019)

PDLIM1 shows up as a clear band at the correct molecular weight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM1/CLP36 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:11674-1-AP)