Anticorps Polyclonal de lapin anti-PDLIM7

• Phare
• Validé par KD/KO

PDLIM7 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

13

Avis (2)

N° de cat : 10221-1-AP

Synonymes

ENIGMA, LIM mineralization protein, LMP, PDLIM7, PDZ and LIM domain 7 (enigma), PDZ and LIM domain protein 7, Protein enigma

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Galerie de données de validation

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  WB analysis of HeLa using 10221-1-AP

  WB result of PDLIM7 antibody (10221-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

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  WB analysis of SKOV-3 using 10221-1-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10221-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10221-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 10221-1-AP (PDLIM7 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa cells using 10221-1-AP

  IP result of PDLIM7 antibody (10221-1-AP for IP and Detection) with HeLa cell lysate.

• 

  IP experiment of MCF-7 using 10221-1-AP

  IP Result of anti-PDLIM7,LMP1 (IP:10221-1-AP, 4ug; Detection:10221-1-AP 1:300) with MCF-7 cells lysate 2000ug.

• 

  IHC staining of human ovary tumor using 10221-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10221-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 25x lens).

• 

  IF Staining of SKOV-3 using 10221-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed SKOV-3 cells using 10221-1-AP (PDLIM7 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules HeLa, cellules MCF-7
• Résultats positifs en IP: cellules HeLa, cellules MCF-7
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules SKOV-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

10221-1-AP cible PDLIM7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDLIM7 Protéine recombinante Ag0264
• Nom complet: PDZ and LIM domain 7 (enigma)
• Masse moléculaire calculée: 55/60 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC001093
• Symbole du gène: PDLIM7
• Identification du gène (NCBI): 9260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.

Publications

Species	Application	Title
mouse	WB	EMBO Rep; Salt-inducible kinase induces cytoplasmic histone deacetylase 4 to promote vascular calcification.; Authors - Alon Abend
human,mouse	WB,IHC,IP	Cell Biosci; TPM2 attenuates progression of prostate cancer by blocking PDLIM7-mediated nuclear translocation of YAP1; Authors - Zonglong Wu
human	IF	J Bone Miner Res; LIM Mineralization Protein-1 Enhances Bone Morphogenetic Protein-2 Mediated Osteogenesis through Activation of ERK1/2 MAPK Pathway and Upregulation of Runx2 Transactivity.; Authors - Hehai Pan
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
human	IHC	J Endod; Immunohistochemical localization of LIM mineralization protein 1 in pulp-dentin complex of human teeth with normal and pathologic conditions.; Authors - Wang Xiaoying X
human	WB	J Orthop Res; LIM mineralization protein-1 suppresses TNF-α induced intervertebral disc degeneration by maintaining nucleus pulposus extracellular matrix production and inhibiting matrix metalloproteinases expression.; Authors - Hui Liu ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This antibody worked quite well in detecting PDLIM7 in mouse heart protein lysate.

• Applications: Western Blot
• Cell Tissue Type: Heart

FH

Joleen (Verified Customer) (06-10-2019)

The most prominent band correspond to where PDLIM7 is expected to be. But there are other surrounding background bands.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1k
• Cell Tissue Type: MDCK GII

PDLIM7 Antibody Western Blot, validation (1:1k dilution) in MDCK GII (Cat no:10221-1-AP)