Anticorps Polyclonal de lapin anti-PDXDC1

PDXDC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 21021-1-AP

Synonymes

Pyridoxal-dependent decarboxylase domain-containing protein 1, LP8165, KIAA0251, EC:4.1.1.-

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Galerie de données de validation

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  WB analysis using 21021-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 21021-1-AP

  mouse testis lysates were subjected to SDS PAGE followed by western blot with 21021-1-AP (PDXDC1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 21021-1-AP

  IP result of anti-PDXDC1 (IP:21021-1-AP, 4ug; Detection:21021-1-AP 1:500) with HepG2 cells lysate 1680 ug.

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21021-1-AP (PDXDC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 21021-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 21021-1-AP (PDXDC1 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 21021-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDXDC1 antibody (21021-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HeLa using 21021-1-AP

  Immunofluorescent analysis of Hela cells, using PDXDC1 antibody 21021-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules SGC-7901, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

21021-1-AP cible PDXDC1 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PDXDC1 Protéine recombinante Ag15283
• Nom complet: pyridoxal-dependent decarboxylase domain containing 1
• Masse moléculaire calculée: 788 aa, 87 kDa
• Poids moléculaire observé: 87 kDa
• Numéro d’acquisition GenBank: BC033748
• Symbole du gène: PDXDC1
• Identification du gène (NCBI): 23042
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PDXDC1 (pyridoxal-dependent decarboxylase domain containing 1) is a putative enzyme that could metabolize catecholamine neurotransmitters (PMID:28485732). With prior evidence for involvement with glioblastoma from other previously reported experimental settings, and contains the lead single nucleotide polymorphism (rs3198697) from the linkage analysis of the chromosome 16 region (PMID:32644145). Moreover, PDXDC1 is involved in the catalysis of the nonhydrolytic addition or removal of a carboxyl group to or from a compound (PMID:29277971).

Publications

Species	Application	Title
mouse	IHC	J Mol Med (Berl); Identification of PDXDC1 as a novel pleiotropic susceptibility locus shared between lumbar spine bone mineral density and birth weight.; Authors - Yu-Qian Song
mouse	WB	Cell Mol Life Sci; Protease-independent control of parthanatos by HtrA2/Omi; Authors - Jonas Weiß
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang

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