Anticorps Polyclonal de lapin anti-CD31

• Phare
• Validé par KD/KO

CD31 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (5)

Applications

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Conjugaison

Non conjugué

Publications

289

N° de cat : 11265-1-AP

Synonymes

CD31, EndoCAM, GPIIA, PECA1, PECAM 1, PECAM1, VEC marker

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Galerie de données de validation

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  WB analysis using 11265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HUVEC using 11265-1-AP

  HUVEC cells were subjected to SDS PAGE followed by western blot with 11265-1-AP (CD31 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11265-1-AP

  IP Result of anti-PECAM1,CD31 (IP:11265-1-AP, 5ug; Detection:11265-1-AP 1:300) with Jurkat cells lysate 4000ug.

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  IHC staining of human liver cancer using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens).

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  IHC staining of human placenta using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:4000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11265-1-AP (CD31 Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human renal cell carcinoma using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11265-1-AP (CD31 Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human gliomas using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 11265-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11265-1-AP (CD31 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HUVEC using 11265-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 11265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 11265-1-AP (CD31 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human endometrial cancer using 11265-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using CD31 antibody (11265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HUVEC using 11265-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug CD31 antibody (11265-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: plaquettes de sang périphérique humain, cellules HUVEC, cellules Jurkat, cellules THP-1
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu d'amygdalite humain, tissu de carcinome à cellules rénales humain, tissu de cirrhose hépatique humain, tissu de gliome humain, tissu placentaire humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HUVEC

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 64 publications below
• IHC: See 127 publications below
• IF: See 128 publications below
• IP: See 1 publications below
• FC: See 2 publications below

Informations sur le produit

11265-1-AP cible CD31 dans les applications de WB, IP, IHC, IF, FC, Cell treatment, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: canin, Chèvre, Humain, Lapin, porc, singe
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CD31 Protéine recombinante Ag1787
• Nom complet: platelet/endothelial cell adhesion molecule
• Masse moléculaire calculée: 83 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: BC022512
• Symbole du gène: PECAM1
• Identification du gène (NCBI): 5175
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Platelet endothelial cell adhesion molecule-1 (PECAM-1, CD31) is a member of the immunoglobulin gene superfamily of cell adhesion molecules. CD31 is a transmembrane glycoprotein that is highly expressed on the surface of the endothelium, making up a large portion of its intracellular junctions. PECAM-1 is also present on the surface of hematopoietic cells and immune cells including platelets, monocytes, neutrophils, natural killer cells, megakaryocytes and some types of T-cell (PMID: 9011572). As well as its role in cell-cell adhesion, PECAM-1 functions as a signaling receptor, and is involved in important physiological events such as nitric oxide production, regulation of T-cell immunity and tolerance, leukocyte transendothelial migration and inflammation and angiogenesis (PMID: 21183735; 20978210; 17872453; 20634489).

Publications

Species	Application	Title
	IHC	Bioact Mater; Li-Mg-Si bioceramics provide a dynamic immuno-modulatory and repair-supportive microenvironment for peripheral nerve regeneration; Authors - Yiting Sun
rabbit	WB,IF	Nat Commun; Four-dimensional hydrogel dressing adaptable to the urethral microenvironment for scarless urethral reconstruction; Authors - Yujie Hua
human	IHC	Sci Transl Med; Selective targeting of multiple myeloma cells with a monoclonal antibody recognizing the ubiquitous protein CD98 heavy chain.; Authors - Kana Hasegawa
human	IHC,IF	Cancer Commun (Lond); Glutamine metabolic microenvironment drives M2 macrophage polarization to mediate trastuzumab resistance in HER2-positive gastric cancer; Authors - Xingbin Hu
human	IHC	Mol Cancer; CircPTPRA blocks the recognition of RNA N6-methyladenosine through interacting with IGF2BP1 to suppress bladder cancer progression.; Authors - Fei Xie
monkey,human	IHC	Acta Pharm Sin B; Nonclinical safety, tolerance and pharmacodynamics evaluation for meplazumab treating chloroquine-resistant Plasmodium falciparum.; Authors - Kun Zhang

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