Anticorps Polyclonal de lapin anti-Serpin F1/PEDF

Serpin F1/PEDF Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 26045-1-AP

Synonymes

PIG35, PEDF, Pigment epithelium-derived factor, EPC-1, EPC 1

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Galerie de données de validation

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  WB analysis of human serum using 26045-1-AP

  human serum were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 26045-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 26045-1-AP (PEDF antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 26045-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 26045-1-AP (PEDF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A375, human serum
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

26045-1-AP cible Serpin F1/PEDF dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Serpin F1/PEDF Protéine recombinante Ag23296
• Nom complet: serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
• Masse moléculaire calculée: 418 aa, 46 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC013984
• Symbole du gène: PEDF
• Identification du gène (NCBI): 5176
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PEDF also known as serpin F1 (SERPINF1), is a multifunctional secreted protein that has anti-angiogenic, anti-tumorigenic, and neurotrophic functionsis. It is an approximately 50-kDa secreted protein that is widely expressed, including by osteoblasts and osteoclasts. PEDF is also one of the most abundant secretory products of adipocytes, and circulating concentrations of PEDF correlate positively with body fat mass and INS resistance. PEDF is a neurotrophic factor involved in neuronal differentiation in retinoblastoma cells. Mutations in this gene were found in individuals with osteogenesis imperfecta, type VI.

Publications

Species	Application	Title
human	WB,IF	J Cell Mol Med; Lycopene inhibits endothelial-to-mesenchymal transition of choroidal vascular endothelial cells in laser-induced mouse choroidal neovascularization; Authors - Lele Li
human	WB	Hum Gene Ther; Strategies to Attenuate Myocardial Infarction and No-Reflow through Preservation of Vascular Integrity by Pigment Epithelium-derived Factor.; Authors - Hao Zhang
human	WB	Cell Death Dis; TFAP2B overexpression contributes to tumor growth and progression of thyroid cancer through the COX-2 signaling pathway.; Authors - Xiaoyan Fu
human,mouse	WB,IHC	Cell Signal; SERPINF1 knockdown attenuates chondrocyte senescence, hypertrophy, and inflammation in osteoarthritis to offer a potential therapeutic strategy; Authors - Lini Dong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Boyan (Verified Customer) (05-14-2021)

very good for WB

• Applications: Western Blot

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