Anticorps Polyclonal de lapin anti-PEG10

PEG10 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(10)

Avis (2)

N° de cat : 14412-1-AP

Synonymes

EDR, Embryonal carcinoma differentiation-regulated protein, HB 1, HB1, KIAA1051

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Galerie de données de validation

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  WB analysis using 14412-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14412-1-AP (PEG10 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skin using 14412-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 14412-1-AP (PEG10 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14412-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEG10 antibody (14412-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules L02, cellules MCF-7, cellules MDA-MB-231, cellules T-47D
• Résultats positifs en IHC: tissu cutané de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

14412-1-AP cible PEG10 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEG10 Protéine recombinante Ag5754
• Nom complet: paternally expressed 10
• Masse moléculaire calculée: 80 kDa
• Poids moléculaire observé: 50 kDa, 100 kDa
• Numéro d’acquisition GenBank: BC050659
• Symbole du gène: PEG10
• Identification du gène (NCBI): 23089
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	IHC,IF	Cell; Cellular architecture of human brain metastases.; Authors - Hugo Gonzalez
human	WB	Acta Pharmacol Sin; Transcriptional regulator CTR9 promotes hepatocellular carcinoma progression and metastasis via increasing PEG10 transcriptional activity.; Authors - Bin Zhang
human	WB,IF	bioRxiv; Endogenous retrovirus-like proteins recruit UBQLN2 to stress granules and alter their functional properties; Authors - Harihar M Mohan
human	WB	Leuk Res; NAP1L5 promotes epithelial-mesenchymal transition by regulating PEG10 expression in acute myeloid leukaemia; Authors - Huan Wu ; KD Validated
human	WB,CoIP	PLoS One; The gag-like gene RTL8 antagonizes PEG10-mediated virus like particles; Authors - Will Campodonico
human	IP,IF	Int J Biol Macromol; Evolutionary dynamics of PEG10 and its interacting proteins during early and late-stage placental development in ruminants; Authors - Yayi Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Harihar (Verified Customer) (01-28-2024)

The antibody is excellent for Western blotting. There is some non-specific nuclear staining in case of IF at the dilutions we have tested at.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:300 (IF), 1:3000 (WB)
• Cell Tissue Type: HeLa cells, Kolf2 iPSCs

PEG10 Antibody Western Blot,Immunofluorescence validation (1:300 (IF), 1:3000 (WB) dilution) in HeLa cells, Kolf2 iPSCs (Cat no:14412-1-AP)

FH

Jordan (Verified Customer) (09-03-2019)

The antibody works well in our condition. MCF-7 cell lines. 1/1000 in TBS-T 5%BSA.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000

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