Anticorps Polyclonal de lapin anti-PELO

• Phare
• Validé par KD/KO

PELO Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

Avis (1)

N° de cat : 10582-1-AP

Synonymes

CGI 17, PELO, pelota homolog (Drosophila), PRO1770, Protein pelota homolog

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Galerie de données de validation

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  WB analysis using 10582-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10582-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10582-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 10582-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10582-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10582-1-AP (PELO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10582-1-AP

  IP Result of anti-PELO (IP:10582-1-AP, 4ug; Detection:10582-1-AP 1:500) with HEK-293 cells lysate 1200ug.

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  IHC staining of human liver using 10582-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 10582-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10582-1-AP (PELO antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS cells using 10582-1-AP

  IF result of 10582-1-AP (PELO antibody) in U2OS cell by Dr. Nancy Kedersha.(cytoplasm and nucleus; Green).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules SKOV-3, tissu testiculaire de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules U2OS

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below

Informations sur le produit

10582-1-AP cible PELO dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PELO Protéine recombinante Ag0873
• Nom complet: pelota homolog (Drosophila)
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43-45 kDa
• Numéro d’acquisition GenBank: BC005889
• Symbole du gène: PELO
• Identification du gène (NCBI): 53918
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

Publications

Species	Application	Title
human	WB	Mol Cell; Unresolved stalled ribosome complexes restrict cell-cycle progression after genotoxic stress.; Authors - Mark Stoneley
human	WB	Nucleic Acids Res; XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.; Authors - Shuai Hou
mouse	WB	Elife; Defects in translation-dependent quality control pathways lead to convergent molecular and neurodevelopmental pathology.; Authors - Markus Terrey
human		Cell Rep; A role for the ribosome-associated complex in activation of the IRE1 branch of UPR.; Authors - I-Hui Wu ; KD Validated
mouse	WB	PLoS Genet; Mammalian Hbs1L deficiency causes congenital anomalies and developmental delay associated with Pelota depletion and 80S monosome accumulation.; Authors - Amy E O'Connell
mouse	WB	Immunity; Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity; Authors - Xiurong Wu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This is a good antibody. It works well in western blot using chemiluminescence but not fluorescent secondary antibodies.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa, MT4, HEK293T

PELO Antibody Western Blot validation (1:1000 dilution) in HeLa, MT4, HEK293T (Cat no:10582-1-AP)