Anticorps Monoclonal anti-PEPD

PEPD Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC

Conjugaison

Non conjugué

Publications

1

CloneNo.

1H2A1

N° de cat : 67202-1-Ig

Synonymes

Imidodipeptidase, PEPD, peptidase D, PRD, PROLIDASE, Proline dipeptidase, X Pro dipeptidase, Xaa Pro dipeptidase

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Galerie de données de validation

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  WB analysis using 67202-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 67202-1-Ig

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 67202-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 67202-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67202-1-Ig (PEPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67202-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67202-1-Ig (PEPD antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules NCCIT, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

67202-1-Ig cible PEPD dans les applications de WB, IHC et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PEPD Protéine recombinante Ag28713
• Nom complet: peptidase D
• Masse moléculaire calculée: 493 aa, 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC015027
• Symbole du gène: PEPD
• Identification du gène (NCBI): 5184
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20 ℃. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PEPD, also named as PRD, Prolidase, X-Pro dipeptidase, Imidodipeptidase, Peptidase D and Proline dipeptidase, belongs to the peptidase M24B family and Eukaryotic-type prolidase subfamily. PEPD splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. It plays an important role in collagen metabolism because the high level of iminoacids in collagen. Defects in PEPD are a cause of prolidase deficiency (PD). PEPD is considered as the most promising candidate genes for altering AAA risk, based on gene function, association evidence, gene expression, and protein expression.(PMID:21247474)

Publications

Species	Application	Title
human	WB	Front Bioeng Biotechnol; Small-Molecule Induction Promotes Corneal Endothelial Cell Differentiation From Human iPS Cells.; Authors - Jie Chen