Anticorps Polyclonal de lapin anti-PEX14

PEX14 Polyclonal Antibody for FC (Intra), IF

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

IF, FC (Intra)

Conjugaison

CoraLite®594 Fluorescent Dye

Publications

6

N° de cat : CL594-10594

Synonymes

Cell and organelle markers, dJ734G22.2, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

---

Galerie de données de validation

• 

  IF Staining of HeLa using CL594-10594

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

• 

  IF Staining of HeLa using CL594-10594

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite®594 PEX14 antibody (CL594-10594) at dilution of 1:400, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green).

• 

  FC experiment of HeLa using CL594-10594

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human PEX14 (CL594-10594) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 6 publications below

Informations sur le produit

CL594-10594 cible PEX14 dans les applications de IF, FC (Intra) et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PEX14 Protéine recombinante Ag0932
• Nom complet: peroxisomal biogenesis factor 14
• Masse moléculaire calculée: 41 kDa
• Poids moléculaire observé: 57 kDa
• Numéro d’acquisition GenBank: BC006327
• Symbole du gène: PEX14
• Identification du gène (NCBI): 5195
• Conjugaison: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec glycérol à 50 %, Proclin300 à 0,05 % et BSA à 0,5 %, pH 7,3.
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

Publications

Species	Application	Title
rat	IF	Mol Neurobiol; Schwann Cell-Derived Exosomes Ameliorate Paclitaxel-Induced Peripheral Neuropathy Through the miR-21-Mediated PTEN Signaling Pathway; Authors - Min You
mouse	IF	Inflamm Res; GCH1 reduces LPS-induced alveolar macrophage polarization and inflammation by inhibition of ferroptosis; Authors - Yuhong Xiao
mouse	IF	J Neuroinflammation; TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy; Authors - Xuliang Huang
human,mouse,rat	IF	Am J Physiol Cell Physiol; OTUD6A in tubular epithelial cells mediates Angiotensin II-induced kidney injury by targeting STAT3; Authors - Xiaoyu Sun
	IF	Pharmaceuticals (Basel); A Class I HDAC Inhibitor BG45 Alleviates Cognitive Impairment through the CaMKII/ITPKA/Ca2+ Signaling Pathway; Authors - Jingyun Liu
mouse	IF	J Ethnopharmacol; TMT quantitative proteomics and network pharmacology reveal the mechanism by which asiaticoside regulates the JAK2/STAT3 signaling pathway to inhibit peritoneal fibrosis; Authors - Jinyi Sun

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C