Anticorps Polyclonal de lapin anti-Pepsinogen I

Pepsinogen I Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 17330-1-AP

Synonymes

PGA5, PGI, Pepsinogen-5, Pepsin A-5, Pepsin A

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Galerie de données de validation

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  WB analysis using 17330-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17330-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17330-1-AP (Pepsinogen I antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 17330-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17330-1-AP (Pepsinogen I antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules BGC-823, cellules SGC-7901
• Résultats positifs en IHC: tissu d'estomac de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

17330-1-AP cible Pepsinogen I dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Pepsinogen I Protéine recombinante Ag11309
• Nom complet: pepsinogen 5, group I (pepsinogen A)
• Masse moléculaire calculée: 388 aa, 42 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC029055
• Symbole du gène: PGA5
• Identification du gène (NCBI): 5222
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pepsinogen I belongs to the peptidase A1 family. It shows particularly broad specificity and although bonds involving phenylalanine and leucine are preferred, many others are also cleaved to some extent. The gene encodes a 388 amino acid protein with a 15 amino acid signal peptide and 46 amino acid propeptide. This antibody can recognize PGA3(pepsinogen 3), PGA4(pepsinogen 4), PGA5(pepsinogen 5).

Publications

Species	Application	Title
		J Pathol; Bmi1 marks gastric stem cells located in the isthmus in mice.; Authors - Takuto Yoshioka
mouse	WB	Biochem Biophys Res Commun; Changed PGA and POSTN levels in choroid plexus are associated with depressive-like behaviors in mice.; Authors - Jie Liu
rat	IF	PLoS One; The cytoprotective protein clusterin is overexpressed in hypergastrinemic rodent models of oxyntic preneoplasia and promotes gastric cancer cell survival.; Authors - Pål Vange
human	IHC	Hum Pathol; Clinical Significance of Spasmolytic Polypeptide-expressing Metaplasia and Intestinal Metaplasia in EBV-associated and EBV-negative Gastric Cancer.; Authors - Yu Zhang
mouse	IHC	Dev Dyn; Glypican-6 and Glypican-4 stimulate embryonic stomach growth by regulating Hedgehog and non-canonical Wnt signaling; Authors - Wen Shi
pig	IHC	J Agric Food Chem; Casein Hydrolysate Enhances Upper Gastrointestinal Chemosensing and Gastric Acid Secretion in Pigs; Authors - Chunlong Mu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

FAISAL (Verified Customer) (05-03-2021)

I found this antibody very good and give very good and consistent result in low concentration.

• Applications: Western Blot, Immunohistochemistry
• Cell Tissue Type: cell and tissue

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