Anticorps Monoclonal anti-PGK2

PGK2 Monoclonal Antibody for WB, IF-P, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF-P, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3D4F2

N° de cat : 68228-1-Ig

Synonymes

PGKPS, PGKB, EC:2.7.2.3, 3D4F2

---

Galerie de données de validation

• 

  WB analysis using 68228-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 68228-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 68228-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 68228-1-Ig (PGK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse testis using 68228-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PGK2 antibody (68228-1-Ig, Clone: 3D4F2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of A549 using 68228-1-Ig

  1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human PGK2 (68228-1-Ig, Clone:3D4F2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HuH-7, cellules MOLT-4, cellules Raji, tissu cardiaque de porc, tissu cardiaque de rat, tissu testiculaire humain
• Résultats positifs en IF-P: tissu testiculaire de souris,
• Résultats positifs en FC (Intra): cellules A549,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68228-1-Ig cible PGK2 dans les applications de WB, IF-P, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PGK2 Protéine recombinante Ag20631
• Nom complet: phosphoglycerate kinase 2
• Masse moléculaire calculée: 417 aa, 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC038843
• Symbole du gène: PGK2
• Identification du gène (NCBI): 5232
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGK2, phosphoglycerate kinase 2, which belongs to the phosphoglycerate kinase superfamily. It catalyzes the first ATP-generating step in the central metabolic pathway of glycolysis, converting 1,3-bisphosphoglycerate and ADP to 3-phosphoglycerate and ATP.

{{ptg:RelatedPrimaryAntibodies}}