Anticorps Polyclonal de lapin anti-PGM2L1

PGM2L1 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 13942-1-AP

Synonymes

BM32A, EC:2.7.1.106, Glucose 1,6-bisphosphate synthase, Phosphoglucomutase-2-like 1, PMMLP

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Galerie de données de validation

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  WB analysis using 13942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 13942-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 13942-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13942-1-AP (PGM2L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13942-1-AP

  IP result of anti-PGM2L1 (IP:13942-1-AP, 4ug; Detection:13942-1-AP 1:300) with mouse brain tissue lysate 4000 ug.

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  IHC staining of human gliomas using 13942-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13942-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13942-1-AP (PGM2L1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu de cervelet de rat, tissu de cervelet de souris, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below

Informations sur le produit

13942-1-AP cible PGM2L1 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PGM2L1 Protéine recombinante Ag5019
• Nom complet: phosphoglucomutase 2-like 1
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC059360
• Symbole du gène: PGM2L1
• Identification du gène (NCBI): 283209
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGM2L1 (Phosphoglucomutase 2 Like 1) is an enzyme-like protein involved in carbohydrate metabolism. PGM2L1 catalyzes the formation of G16BP by using the glycolytic intermediate 1,3-bisphosphoglycerate as a phosphate donor and glucose-1-phosphate or glucose-6-phosphate as a phosphate acceptor. PGM2L1 plays a crucial role in glycogen metabolism, glycolysis, and gluconeogenesis as a key enzyme, and increasing evidence links its function to cancer metabolism and progression. While PGM2 has a wide tissue distribution, PGM2L1 is highly expressed in the brain, accounting for the elevated concentrations of glucose-1,6-bisphosphate found there (PMID: 33979636, PMID: 40027181).

Publications

Species	Application	Title
human	WB,IHC	Mol Metab; Glucose-1,6-bisphosphate: a new gatekeeper of cerebral mitochondrial pyruvate uptake; Authors - Motahareh Solina Safari ; KD Validated
mouse	WB	Metabolism; Protein kinase N1 deficiency results in upregulation of cerebral energy metabolism and is highly protective in in vivo and in vitro stroke models; Authors - Stephanie Zur Nedden
human	WB,IHC	Cell Death Dis; Cancer-associated fibroblasts secrete CSF3 to promote TNBC progression via enhancing PGM2L1-dependent glycolysis reprogramming; Authors - Wenqi Qin

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