Anticorps Polyclonal de lapin anti-PGP

PGP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 25081-1-AP

Synonymes

Aspartate-based ubiquitous Mg(2+)-dependent phosphatase, AUM, EC:3.1.3.21, EC:3.1.3.48, G3PP



Applications testées

Résultats positifs en WBcellules HepG2, cellules K-562, cellules SH-SY5Y, cellules Y79, tissu cardiaque de souris
Résultats positifs en IPcellules Y79
Résultats positifs en IHCtissu cardiaque de souris, tissu d'amygdalite humain, tissu de muscle squelettique humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules MCF-7,
Résultats positifs en FC (Intra)cellules SH-SY5Y,

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

25081-1-AP cible PGP dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PGP Protéine recombinante Ag18623
Nom complet phosphoglycolate phosphatase
Masse moléculaire calculée 321 aa, 34 kDa
Poids moléculaire observé 34 kDa
Numéro d’acquisition GenBankBC157035
Symbole du gène PGP
Identification du gène (NCBI) 283871
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGP(phosphoglycolate phosphatase) belongs to the HAD-like hydrolase superfamily. It is an essential intermediate in the biosynthetic pathway of cardiolipin and a mitochondrial-specific phospholipid regulating the membrane integrity and activities of the organelle. PGP catalyzes the formation of phosphatidylglycerol from phosphatidylglycerophosphate.

Protocole

Product Specific Protocols
WB protocol for PGP antibody 25081-1-APDownload protocol
IHC protocol for PGP antibody 25081-1-APDownload protocol
IF protocol for PGP antibody 25081-1-APDownload protocol
IP protocol for PGP antibody 25081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Acta Biomater

Systematic evaluation of multifunctional paclitaxel-loaded polymeric mixed micelles as a potential anticancer remedy to overcome multidrug resistance.

Authors - Jiulong Zhang
humanIHC

Front Cell Dev Biol

Predicting Panel of Metabolism and Immune-Related Genes for the Prognosis of Human Ovarian Cancer.

Authors - Lingyun Zhang
humanWB

Chin Med

The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters

Authors - Wenjie Huang
humanWB,IF

J Nanobiotechnology

Combination of polythyleneimine regulating autophagy prodrug and Mdr1 siRNA for tumor multidrug resistance

Authors - Changduo Wang
humanWB

Eur J Med Chem

Design, synthesis and biological evaluation of novel phenylfuran-bisamide derivatives as P-glycoprotein inhibitors against multidrug resistance in MCF-7/ADR cell

Authors - Zhikun Yang
ratIF

J Peripher Nerv Syst

Effect of age on metabolomic changes in a model of paclitaxel-induced peripheral neurotoxicity

Authors - Roberta Bonomo

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kerstin (Verified Customer) (10-11-2024)

nice detection of PGP in HT1080 WT cells compared to KO cells, few unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: HT1080
PGP Antibody Western Blot validation (1:5000 dilution) in HT1080 (Cat no:25081-1-AP)
FH

Yu Siong (Verified Customer) (10-13-2019)

3 protein bands at 150 kDa are of rat liver whole tissue lysate.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Rat liver tissue
PGP Antibody Western Blot, validation (1:500 dilution) in Rat liver tissue (Cat no:25081-1-AP)
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