Anticorps Polyclonal de lapin anti-Prohibitin 2

• Phare
• Validé par KD/KO

Prohibitin 2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IP, IHC, IF, FC, Electrochemical Immunosensor, CoIP, ELISA

Conjugaison

Non conjugué

Publications

40

N° de cat : 12295-1-AP

Synonymes

BAP, Bap37, D prohibitin, p22, PHB2, PNAS 141, prohibitin 2, REA

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Galerie de données de validation

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  WB analysis using 12295-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12295-1-AP

  WB result of Prohibitin 2 antibody (12295-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Prohibitin 2 transfected HeLa cells.

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  IP experiment of HeLa using 12295-1-AP

  IP Result of anti-Prohibitin 2 (IP:12295-1-AP, 3ug; Detection:12295-1-AP 1:1000) with HeLa cells lysate 1840ug.

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  IP experiment of mouse brain using 12295-1-AP

  IP Result of anti-Prohibitin 2 (IP:12295-1-AP, 4ug; Detection:12295-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 12295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12295-1-AP (Prohibitin 2 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 12295-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12295-1-AP (Prohibitin 2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HeLa using 12295-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Prohibitin 2 (12295-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IP: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 35 publications below
• IHC: See 3 publications below
• IF: See 7 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

12295-1-AP cible Prohibitin 2 dans les applications de WB, IP, IHC, IF, FC, Electrochemical Immunosensor, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, riz, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Prohibitin 2 Protéine recombinante Ag2977
• Nom complet: prohibitin 2
• Masse moléculaire calculée: 299 aa, 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC014766
• Symbole du gène: PHB2
• Identification du gène (NCBI): 11331
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHB2, also named as BAP, REA, D-prohibitin, BAP37 and Prohibitin-2, belongs to the prohibitin family. It acts as a mediator of transcriptional repression by nuclear hormone receptors via recruitment of histone deacetylases. PHB2 functions as an estrogen receptor (ER)-selective coregulator that potentiates the inhibitory activities of antiestrogens and represses the activity of estrogens. It competes with NCOA1 for modulation of ER transcriptional activity. PHB2 is probably involved in regulating mitochondrial respiration activity and in aging.(PMID: 10359819)

Publications

Species	Application	Title
human	WB	J Am Soc Nephrol; Bif-1 Interacts with Prohibitin-2 to Regulate Mitochondrial Inner Membrane during Cell Stress and Apoptosis.; Authors - Sung-Gyu Cho
mouse	WB	Neuron; Oligodendrocytes enhance axonal energy metabolism by deacetylation of mitochondrial proteins through transcellular delivery of SIRT2.; Authors - Kelly A Chamberlain
rat	IF	Nat Commun; Spatial mapping of juxtacrine axo-glial interactions identifies novel molecules in peripheral myelination.; Authors - Y Poitelon
mouse		Redox Biol; PHB2 ameliorates Doxorubicin-induced cardiomyopathy through interaction with NDUFV2 and restoration of mitochondrial complex I function; Authors - Mingjie Yang
human	WB	Autophagy; PHB2 (prohibitin 2) promotes PINK1-PRKN/Parkin-dependent mitophagy by the PARL-PGAM5-PINK1 axis.; Authors - Chaojun Yan ; KD Validated
human	WB	EMBO J; ATAD3B is a mitophagy receptor mediating clearance of oxidative stress-induced damaged mitochondrial DNA.; Authors - Li Shu ; KD Validated