Anticorps Polyclonal de lapin anti-PHF19

• Phare
• Validé par KD/KO

PHF19 Polyclonal Antibody for IF, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 11895-1-AP

Synonymes

hPCL3, MTF2L1, PCL3, PHD finger protein 19, PHF19, Polycomb like protein 3

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Galerie de données de validation

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  WB analysis of rat brain using 11895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 11895-1-AP (PHF19 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of Mouse ES cells using 11895-1-AP

  IF result of Mouse ES cell From Kevin Corbit.

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat,
• Résultats positifs en IF: cellules SE de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

11895-1-AP cible PHF19 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF19 Protéine recombinante Ag2511
• Nom complet: PHD finger protein 19
• Masse moléculaire calculée: 580 aa, 63 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC022374
• Symbole du gène: PHF19
• Identification du gène (NCBI): 26147
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHD FINGER PROTEIN 19(PHF19), a protein homologous to the Drosophila 'Polycomblike' (Pcl) protein, encoded by human gene PCL, which encodes 2 deduced proteins: a short form of 207 aa and a long form of 580 aa. The PCL family proteins contains a PCL homology domain, a Tudor domain, 2 PHD fingers and a C-terminal tail. It is highly expressed in thymus and heart, while 508aa form predominant in placenta, skeletal muscle and kidney and 207aa form in liver and peripheral blood leukocytes. Both of these forms can inhibit transcriptional activity. And the long form is corelated with cancer.Acts as a transcritpional repressor. The mRNA level of PHF19 is low in many normal tissue, it's elevated in cancer-derived cell lines.

Publications

Species	Application	Title
mouse	WB	Mol Cell; An H3K36 methylation-engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting.; Authors - Ling Cai ; KD Validated
human		PLoS Genet; Alu Elements in ANRIL Non-Coding RNA at Chromosome 9p21 Modulate Atherogenic Cell Functions through Trans-Regulation of Gene Networks.; Authors - Holdt Lesca M LM
mouse	WB	PLoS Genet; Polycomb-like 3 promotes polycomb repressive complex 2 binding to CpG islands and embryonic stem cell self-renewal.; Authors - Julie Hunkapiller ; KD Validated
human	IHC	Front Immunol; Comprehensive Pan-Cancer Genomic Analysis Reveals PHF19 as a Carcinogenic Indicator Related to Immune Infiltration and Prognosis of Hepatocellular Carcinoma.; Authors - Zheng-Yi Zhu
human,mouse	WB	J Cancer; ZNF750 exerted its Antitumor Action in Oral Squamous Cell Carcinoma by regulating E2F2.; Authors - Hong-Li Yang
human	WB,IHC	Scand J Gastroenterol; Downregulation of PHF19 inhibits cell growth and migration in gastric cancer.; Authors - Haixiao Wang ; KD Validated