Anticorps Polyclonal de lapin anti-PHF6

PHF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 51062-1-AP

Synonymes

BORJ, CENP-31, PHD-like zinc finger protein, PHF 6

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Galerie de données de validation

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  WB analysis using 51062-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51062-1-AP (PHF6 antibody) at dilution of 1:64000 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 51062-1-AP (PHF6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 51062-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 51062-1-AP (PHF6 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 51062-1-AP (PHF6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 51062-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PHF6 antibody (51062-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu placentaire humain, tissu cérébral de souris, tissu de cancer de l'estomac humain, tissu de cancer du col de l'utérus humain, tissu de tumeur ovarienne humain, tissu d'estomac de rat, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:20000-1:100000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

51062-1-AP cible PHF6 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHF6 Protéine recombinante Ag2102
• Nom complet: PHD finger protein 6
• Masse moléculaire calculée: 365aa,41 kDa; 312aa,35 kDa
• Poids moléculaire observé: 41 kDa
• Numéro d’acquisition GenBank: BC005994
• Symbole du gène: PHF6
• Identification du gène (NCBI): 84295
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHF6 protein is highly conserved in vertebrates. Structurally, PHF6 protein contains two plant homeodomain (PHD)-like zinc fingers, two nuclear localization sequences and a nucleolar localization sequence, suggesting that PHF6 may play a role in the regulation of transcription. PHD zinc fingers are structurally conserved modules that interact with chromatin or mediate protein-protein interactions. PHF6 associates with subunits of the PAF1 transcription elongation complex including PAF1, LEO1, CTR9 and CDC73.The interaction of PHF6 with the PAF1 complex plays a critical role in PHF6 function in neuronal migration in the cerebral cortex.

Publications

Species	Application	Title
human	WB	Leuk Res; MiR-1306-5p promotes cell proliferation and inhibits cell apoptosis in acute myeloid leukemia by downregulating PHF6 expression.; Authors - Xiang Gao

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