Anticorps Polyclonal de lapin anti-PHLDA2

• Phare
• Validé par KD/KO

PHLDA2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(7)

N° de cat : 14661-1-AP

Synonymes

BRW1C, BWR1C, HLDA2, IPL, p17 BWR1C, PHLDA2, TSSC3

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Galerie de données de validation

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  WB analysis using 14661-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 14661-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14661-1-AP

  WB result of PHLDA2 antibody (14661-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLDA2 transfected MCF-7 cells.

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  WB analysis of PC-3 using 14661-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14661-1-AP (PHLDA2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 14661-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 14661-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14661-1-AP (PHLDA2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules L02, cellules MCF-7, cellules PC-3, tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer de la prostate humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 2 publications below

Informations sur le produit

14661-1-AP cible PHLDA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Chèvre, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHLDA2 Protéine recombinante Ag6331
• Nom complet: pleckstrin homology-like domain, family A, member 2
• Masse moléculaire calculée: 17 kDa
• Poids moléculaire observé: 20-25 kDa
• Numéro d’acquisition GenBank: BC005034
• Symbole du gène: PHLDA2
• Identification du gène (NCBI): 7262
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.; Authors - Yuji Kubota
human	WB	Aging (Albany NY); PHLDA2 regulates EMT and autophagy in colorectal cancer via the PI3K/AKT signaling pathway.; Authors - Zhan Ma ; KD Validated
human	WB	Pathol Res Pract; FOSL1 transcriptionally regulates PHLDA2 to promote 5-FU resistance in colon cancer cells; Authors - Guangyi Liu ; KD Validated
Goat	WB	Gene; Inhibition of PHLDA2 transcription by DNA methylation and YY1 in goat placenta.; Authors - Ruihong Wang
human	IHC,WB	Sci Rep; Characteristics of lactate metabolism phenotype in hepatocellular carcinoma; Authors - Jiacheng Zhang
human	WB	Cell Metab; PHLDA2-mediated phosphatidic acid peroxidation triggers a distinct ferroptotic response during tumor suppression; Authors - Xin Yang

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