Anticorps Polyclonal de lapin anti-PHLPPL

• Phare
• Validé par KD/KO

PHLPPL Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

Avis (1)

N° de cat : 25244-1-AP

Synonymes

KIAA0931, PHLPP like, PHLPP2, PHLPPL

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Galerie de données de validation

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  WB analysis using 25244-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPPL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25244-1-AP

  WB result of PHLPPL antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPPL transfected HeLa cells.

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  IP experiment of HeLa using 25244-1-AP

  IP result of anti-PHLPPL(IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug.

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  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon cancer using 25244-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPPL Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HeLa using 25244-1-AP

  Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPPL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3
• Résultats positifs en IP: cellules HeLa,
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Informations sur le produit

25244-1-AP cible PHLPPL dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PHLPPL Protéine recombinante Ag19000
• Nom complet: PH domain and leucine rich repeat protein phosphatase-like
• Masse moléculaire calculée: 1323 aa, 147 kDa
• Poids moléculaire observé: 147 kDa
• Numéro d’acquisition GenBank: BC129927
• Symbole du gène: PHLPPL
• Identification du gène (NCBI): 23035
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PHLPPL also named as KIAA0931 or PHLPP2 is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.

Publications

Species	Application	Title
mouse	WB,IF	Oxid Med Cell Longev; Colonic Epithelial PHLPP2 Deficiency Promotes Colonic Epithelial Pyroptosis by Activating the NF-κB Signaling Pathway.; Authors - De-Feng Li ; KD Validated
human,mouse	WB,IF	Mol Ther Nucleic Acids; Tumor-Derived EV-Encapsulated miR-181b-5p Induces Angiogenesis to Foster Tumorigenesis and Metastasis of ESCC.; Authors - Ying Wang
human	WB	Oncotarget; PHLPP negatively regulates cell motility through inhibition of Akt activity and integrin expression in pancreatic cancer cells.; Authors - Alena J Smith ; KD Validated
human	WB,IHC	Oncotarget; High PHLPP1 expression levels predicts longer time of acquired resistance to EGFR tyrosine kinase inhibitors in patients with lung adenocarcinoma.; Authors - Youyou Xie
zebrafish	WB	FASEB J; Role of epigenetic m6 A RNA methylation in vascular development: mettl3 regulates vascular development through PHLPP2/mTOR-AKT signaling.; Authors - Ramendu Parial
human	WB, IHC	Front Oncol; Reduced PHLPP Expression Leads to EGFR-TKI Resistance in Lung Cancer by Activating PI3K-AKT and MAPK-ERK Dual Signaling.; Authors - Wei Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Slavica (Verified Customer) (10-04-2021)

The antibody worked very well!

• Applications: Western Blot
• Cell Tissue Type: SHED patient cell lines