Anticorps Polyclonal de lapin anti-PIGO

PIGO Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 16369-1-AP

Synonymes

UNQ632/PRO1249, RP11 182N22.4, PIG-O, PIG O, Phosphatidylinositol-glycan biosynthesis class O protein

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Galerie de données de validation

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  WB analysis of HeLa using 16369-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16369-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 16369-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16369-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16369-1-AP (PIGO antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16369-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16369-1-AP (PIGO antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of MCF-7 using 16369-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PIGO antibody 16369-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules COLO 320, tissu splénique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu cutané humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16369-1-AP cible PIGO dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIGO Protéine recombinante Ag9232
• Nom complet: phosphatidylinositol glycan anchor biosynthesis, class O
• Masse moléculaire calculée: 1089aa,119 kDa; 454aa,50 kDa
• Poids moléculaire observé: 74 kDa
• Numéro d’acquisition GenBank: BC013987
• Symbole du gène: PIGO
• Identification du gène (NCBI): 84720
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
Mouse	WB	Oxid Med Cell Longev; Nrf2 Activation Attenuates Chronic Constriction Injury-Induced Neuropathic Pain via Induction of PGC-1α-Mediated Mitochondrial Biogenesis in the Spinal Cord.; Authors - Jia Sun
Mouse		CNS Neurosci Ther; Attenuation of neuronal ferroptosis in intracerebral hemorrhage by inhibiting HDAC1/2: Microglial heterogenization via the Nrf2/HO1 pathway; Authors - Zhiwen Jiang
		Br J Pharmacol; Inhibition of Brd4 alleviates osteoarthritis pain via suppression of neuroinflammation and activation of Nrf2-mediated antioxidant signalling; Authors - Jia Sun

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