Anticorps Polyclonal de lapin anti-PIK3C2A

• Phare
• Validé par KD/KO

PIK3C2A Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

10

Avis (1)

N° de cat : 22028-1-AP

Synonymes

CPK, PI 3 Kinase Class 2A, PI 3-kinase C2alpha, PI3 K C2(ALPHA), PI3 K C2A, PI3K C2 alpha, PIK3C2A

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Galerie de données de validation

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  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 22028-1-AP

  WB result of PIK3C2A antibody (22028-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PIK3C2A transfected HeLa cells.

• 

  WB analysis of HeLa using 22028-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 22028-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22028-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22028-1-AP (PIK3C2A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of PC-3 using 22028-1-AP

  IP result of anti-PIK3C2A (IP:22028-1-AP, 4ug; Detection:22028-1-AP 1:1000) with PC-3 cells lysate 1760 ug.

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  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22028-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22028-1-AP (PIK3C2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 22028-1-AP

  Immunofluorescent analysis of HeLa cells using 22028-1-AP (PIK3C2A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, tissu placentaire humain
• Résultats positifs en IP: cellules PC-3,
• Résultats positifs en IHC: tissu ovarien de souris, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Informations sur le produit

22028-1-AP cible PIK3C2A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIK3C2A Protéine recombinante Ag16894
• Nom complet: phosphoinositide-3-kinase, class 2, alpha polypeptide
• Masse moléculaire calculée: 1686 aa, 191 kDa
• Poids moléculaire observé: 191 kDa
• Numéro d’acquisition GenBank: BC113658
• Symbole du gène: PIK3C2A
• Identification du gène (NCBI): 5286
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Science; PI(3,4)P2-mediated cytokinetic abscission prevents early senescence and cataract formation.; Authors - Federico Gulluni ; KD Validated
human	WB, IF	Cancer Cell; Mitotic Spindle Assembly and Genomic Stability in Breast Cancer Require PI3K-C2α Scaffolding Function.; Authors - Federico Gulluni
rat	WB,IF	Cell Res; Phosphatidylinositol 3,4-bisphosphate regulates neurite initiation and dendrite morphogenesis via actin aggregation.; Authors - Shu-Xin Zhang
human	WB,IF	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization; Authors - Ellis L Ryan ; KD Validated
human	IF, WB	J Cell Sci; Defining endogenous TACC3-chTOG-clathrin-GTSE1 interactions at the mitotic spindle using induced relocalization.; Authors - Ellis L Ryan
human	WB	Front Cell Infect Microbiol; The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.; Authors - Teng Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

HUAYI (Verified Customer) (01-17-2020)

This antibody provide brilliant Western Blot results, and as well, the specificity for Immunofloruorence is high. But we are glad if more discounts would be received.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: cancer cell lines

PIK3C2A Antibody Western Blot,Immunofluorescence, validation (1:1000 dilution) in cancer cell lines (Cat no:22028-1-AP)