Anticorps Monoclonal anti-PI3 Kinase p110 Alpha

• Phare
• Validé par KD/KO

PI3 Kinase p110 Alpha Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, Lapin, Lapin, porc, rat, souris et plus (3)

Applications

WB, RIP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

79

CloneNo.

1F6A7

Avis (1)

N° de cat : 67071-1-Ig

Synonymes

p110 alpha, PI3 Kinase p110 Alpha, PI3 kinase subunit alpha, PI3K, PI3K alpha, PI3K p110 (alpha), PI3K p110(alpha), PIK3CA, PtdIns 3 kinase subunit alpha

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Galerie de données de validation

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  WB analysis of U2OS using 67071-1-Ig

  U2OS cells were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PI3 Kinase p110 Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

• 

  WB analysis of pig brain using 67071-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67071-1-Ig (PIK3CA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 67071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 67071-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67071-1-Ig (PIK3CA antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 67071-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PI3 Kinase p110 Alpha antibody (67071-1-Ig, Clone: 1F6A7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  FC experiment of Jurkat using 67071-1-Ig

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human PI3 Kinase p110 Alpha (67071-1-Ig, Clone:1F6A7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 76 publications below
• IHC: See 3 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

67071-1-Ig cible PI3 Kinase p110 Alpha dans les applications de WB, RIP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, Lapin, porc, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris, fish
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PI3 Kinase p110 Alpha Protéine recombinante Ag17392
• Nom complet: phosphoinositide-3-kinase, catalytic, alpha polypeptide
• Masse moléculaire calculée: 1068 aa, 124 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC113601
• Symbole du gène: PIK3CA
• Identification du gène (NCBI): 5290
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIK3CA belongs to the PI3/PI4-kinase family. It phosphorylates PtdIns, PtdIns4P and PtdIns(4,5)P2 with a preference for PtdIns(4,5)P2. Defects in PIK3CA are associated with colorectal cancer (CRC). Defects in PIK3CA are associated with breast cancer. Defects in PIK3CA are associated with ovarian cancer. Defects in PIK3CA may underlie hepatocellular carcinoma (HCC). Defects in PIK3CA are a cause of keratosis seborrheic (KERSEB).

Publications

Species	Application	Title
human	WB	J Hazard Mater; Chlorine disinfection elevates the toxicity of polystyrene microplastics to human cells by inducing mitochondria-dependent apoptosis.; Authors - Jing Qin
mouse	IHC	Cancer Lett; PI3K inhibitor impairs tumor progression and enhances sensitivity to anlotinib in anlotinib-resistant osteosarcoma.; Authors - Chenglong Chen
human	WB	Oxid Med Cell Longev; Transcriptional Patterns of Lower-Grade Glioma Patients with Distinct Ferroptosis Levels, Immunotherapy Response, and Temozolomide Sensitivity.; Authors - Zewei Tu
mouse	WB	Oxid Med Cell Longev; Microbiota from Exercise Mice Counteracts High-Fat High-Cholesterol Diet-Induced Cognitive Impairment in C57BL/6 Mice; Authors - Rui Li
human	WB	Anal Chem; Ionic Liquid-Based Extraction System for In-Depth Analysis of Membrane Protein Complexes.; Authors - Hongwei Chu
rat	WB	Sci Total Environ; PKCα mediated by the PI3K/Akt-FOXA1 cascade facilitates cypermethrin-induced hyperthyroidism.; Authors - Mei Ha

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Iru (Verified Customer) (02-18-2021)

Not sure what the band is, I saw a clear band in between 38 and 50KD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Fallopian tube epithelial cells

PI3 Kinase p110 Alpha Antibody Western Blot validation (1:2000 dilution) in Fallopian tube epithelial cells (Cat no:67071-1-Ig)