Anticorps Polyclonal de lapin anti-PIN4

• Phare
• Validé par KD/KO

PIN4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 15789-1-AP

Synonymes

Peptidyl-prolyl cis/trans isomerase EPVH, Parvulin-17, Parvulin-14, hPar14, EPVH

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Galerie de données de validation

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  WB analysis of mouse liver using 15789-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 15789-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15789-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15789-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15789-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15789-1-AP (PIN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15789-1-AP (PIN4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15789-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15789-1-AP (PIN4 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15789-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PIN4 antibody (15789-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HeLa, tissu cérébral de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below

Informations sur le produit

15789-1-AP cible PIN4 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PIN4 Protéine recombinante Ag8489
• Nom complet: protein (peptidylprolyl cis/trans isomerase) NIMA-interacting, 4 (parvulin)
• Masse moléculaire calculée: 131 aa, 14 kDa
• Poids moléculaire observé: 14 kDa
• Numéro d’acquisition GenBank: BC005234
• Symbole du gène: PIN4
• Identification du gène (NCBI): 5303
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PIN4, also known as EPVH, hPar14, is a member of the parvulin subfamily of the peptidyl-prolyl cis/trans isomerase protein family. PIN4 is the first eukaryotic member of the parvulin family of PPIases and contains a PPIase domain preceded by a 40-amino acid glycine- and lysine-rich N-terminal sequence.

Publications

Species	Application	Title
human	WB	Cancer Med; Par14 interacts with the androgen receptor, augmenting both its transcriptional activity and prostate cancer proliferation; Authors - Miki Naito ; KD Validated

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