Anticorps Polyclonal de lapin anti-PISD

• Phare
• Validé par KD/KO

PISD Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 16401-1-AP

Synonymes

PSSC, PSDC, Phosphatidylserine decarboxylase proenzyme, mitochondrial, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase alpha chain

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Galerie de données de validation

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  WB analysis of K-562 using 16401-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 16401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 16401-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16401-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 16401-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 16401-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 16401-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16401-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16401-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16401-1-AP (PISD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 16401-1-AP

  IP result of anti-PISD (IP:16401-1-AP, 3ug; Detection:16401-1-AP 1:500) with L02 cells lysate 1400ug.

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  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 16401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16401-1-AP (PISD antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules HepG2, cellules K-562, cellules L02, cellules NIH/3T3, tissu rénal humain, tissu splénique humain
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

16401-1-AP cible PISD dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PISD Protéine recombinante Ag9499
• Nom complet: phosphatidylserine decarboxylase
• Masse moléculaire calculée: 375aa,43 kDa; 408aa,46 kDa
• Poids moléculaire observé: 45~55 kDa
• Numéro d’acquisition GenBank: BC001482
• Symbole du gène: PISD
• Identification du gène (NCBI): 23761
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.This antibody is specific to PISD. The sequence correspond to the beta isoform.

Publications

Species	Application	Title
human	WB	Cell Death Dis; Aberrant ER-mitochondria communication is a common pathomechanism in mitochondrial disease; Authors - Patricia Morcillo
human	WB	Cancer Lett; LACTB suppresses melanoma progression by attenuating PP1A and YAP interaction.; Authors - Yawen Ma
human		Front Cell Dev Biol; Screening and diagnosis of triple negative breast cancer based on rapid metabolic fingerprinting by conductive polymer spray ionization mass spectrometry and machine learning; Authors - Yaoyao Song
human	WB	J Clin Biochem Nutr; Metabolic changes induced by TGF-β1 via reduced expression of phosphatidylserine decarboxylase during myofibroblast transition; Authors - Nobuhiko Uchida
mouse	WB	bioRxiv; Distinct yet Overlapping Functions of VMP1 and TMEM41B in Modulating Hepatic Lipoprotein Secretion and Autophagy in MASH; Authors - Allen Chen
human	WB	Cell Stem Cell; The Mitochondrial Transacylase, Tafazzin, Regulates for AML Stemness by Modulating Intracellular Levels of Phospholipids.; Authors - Ayesh K Seneviratne ; KO Validated

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