Anticorps Monoclonal anti-PKM2-specific

• Phare
• Validé par KD/KO

PKM2-specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(74)

CloneNo.

1C11C7

Avis (1)

N° de cat : 60268-1-Ig

Synonymes

PKM2, PK-M2, PKM2 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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Galerie de données de validation

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  WB analysis of HEK-293 using 60268-1-Ig

  WB result of PKM2 antibody (60268-1-Ig, 1:3000) with si-Control and si-PKM2 transfected HEK293 cells.

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  WB analysis using 60268-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of MCF-7 using 60268-1-Ig

  Western blot analysis of PK-M2-specific in various lines using Proteintech antibody 60268-1-Ig at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60268-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 60268-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 60268-1-Ig (PKM2-specific antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 60268-1-Ig

  IP result of anti-PKM2-specific (IP:60268-1-Ig, 5ug; Detection:15822-1-AP (PKM2-specific rabbit poly-antibody), 1:300) with HeLa cells lysate 2800ug.

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  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60268-1-Ig (PKM2-specific antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 10x lens).

• 

  IHC staining of human colon cancer using 60268-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:500 (under 40x lens).

• 

  IF Staining of HeLa using 60268-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKM2-specific antibody (60268-1-Ig, Clone: 1C11C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 60268-1-Ig

  Immunofluorescent analysis of HepG2 cells using 60268-1-Ig(PKM2-specific antibody) at dilution of 1:50 and and Rhodamine-labeled goat anti-mouse IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 4T1, cellules C6, cellules HepG2, cellules HSC-T6, cellules K-562, cellules MCF-7, cellules NIH/3T3, cellules ROS1728
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:30000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 63 publications below
• IHC: See 12 publications below
• IF: See 27 publications below
• IP: See 8 publications below
• CoIP: See 6 publications below

Informations sur le produit

60268-1-Ig cible PKM2-specific dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_002654
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms,the activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The immunogen of this antibody is M2 isoform and this antibody is specific to PKM2 isoform.

Publications

Species	Application	Title
human	WB	Adv Mater; Dual-Targeted Novel Temozolomide Nanocapsules Encapsulating siPKM2 Inhibit Aerobic Glycolysis to Sensitize Glioblastoma to Chemotherapy; Authors - Yongkang Zhang
mouse	WB	Cell Metab; Augmentation of scleral glycolysis promotes myopia through histone lactylation; Authors - Xiaolei Lin
human	WB,IP,IHC,IF	Nat Commun; MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration; Authors - Jianxiong Chen
human	WB,IF	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu
rat,mouse	WB,IF	Acta Pharm Sin B; Protocatechuic aldehyde protects cardiomycoytes against ischemic injury via regulation of nuclear pyruvate kinase M2.; Authors - Xunxun Wu ; KD Validated
human	IF	J Exp Clin Cancer Res; NONO interacts with nuclear PKM2 and directs histone H3 phosphorylation to promote triple-negative breast cancer metastasis; Authors - Qixiang Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tianyi (Verified Customer) (01-12-2024)

Antigen retrieval in TE buffer, and incubated with secondary antibody AF647 at room temperature for 2 h.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Ovarian tissue

PKM2-specific Antibody Immunofluorescence validation (1:200 dilution) in Ovarian tissue (Cat no:60268-1-Ig)

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