Anticorps Polyclonal de lapin anti-PKC Beta

• Phare
• Validé par KD/KO

PKC Beta Polyclonal Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

N° de cat : CL488-12919

Synonymes

PRKCB, PKC-beta, PKC-B, PKCB, PKC B

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Galerie de données de validation

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  IF Staining of K-562 using CL488-12919

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using CoraLite® Plus 488 PKC Beta antibody (CL488-12919) at dilution of 1:200.

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  FC experiment of K-562 using CL488-12919

  1X10^6 K-562 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human PKC Beta (CL488-12919) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en IF/ICC: cellules K-562,
• Résultats positifs en FC (Intra): cellules K-562,

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-12919 cible PKC Beta dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Beta Protéine recombinante Ag3591
• Nom complet: protein kinase C, beta
• Masse moléculaire calculée: 673 aa, 77 kDa
• Numéro d’acquisition GenBank: BC036472
• Symbole du gène: PRKCB
• Identification du gène (NCBI): 5579
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. Protein kinase Cs (PKCs) are signaling molecules that play key roles in many cellular processes, including secretion, gene expression, proliferation, and muscle contraction. The PKC family is broadly divided into three subgroups: classical, novel, and atypical PKCs. These subgroups differ in both protein sequences and mechanistic requirements for catalytic activity. PKC beta is one of the PKC family members. PKC beta has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. This antibody can recognize PKC beta, PKC alpha, PKC gamma, PKC epsilon and PKC delta.

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