Anticorps Polyclonal de lapin anti-PKLR

PKLR Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 17580-1-AP

Synonymes

EC:2.7.1.40, PK1, PKL, Pyruvate kinase isozymes L/R, Pyruvate kinase PKLR

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Galerie de données de validation

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  WB analysis of K-562 using 17580-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 17580-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17580-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17580-1-AP (PKLR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 17580-1-AP

  IP result of anti-PKLR (IP:17580-1-AP, 4ug; Detection:17580-1-AP 1:500) with K-562 cells lysate 3200ug.

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  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 17580-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17580-1-AP (PKLR antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules K-562, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

17580-1-AP cible PKLR dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKLR Protéine recombinante Ag11096
• Nom complet: pyruvate kinase, liver and RBC
• Masse moléculaire calculée: 574 aa, 62 kDa, 585 aa, 63 kDa
• Poids moléculaire observé: 58-62 kDa
• Numéro d’acquisition GenBank: BC025737
• Symbole du gène: PKLR
• Identification du gène (NCBI): 5313
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKLR(Pyruvate kinase isozymes R/L) is also named as PK1,PKL,which is a glycolytic enzyme that catalyzes the transphosphorylation from phosphoenolpyruvate (PEP) to ADP, yielding pyruvate and ATP. It is the last step of the glycolytic pathway and is essentially irreversible.It belongs to the pyruvate kinase family and There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. L type is major isozyme in the liver, R is found in red cells, M1 is the main form in muscle, heart and brain, and M2 is found in early fetal tissues.Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) and pyruvate kinase deficiency of red cells (PKRD).It can form a homotetramer(PMID:11960989).

Publications

Species	Application	Title
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang
human	WB	ACS Pharmacol Transl Sci; All Blood Brain Barrier Cell Types Demonstrate Capability to Influence Differential Tenofovir and Emtricitabine Metabolism and Transport in the Brain; Authors - Hannah N Wilkins

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