Anticorps Polyclonal de lapin anti-PKM1-specific

• Phare
• Validé par KD/KO

PKM1-specific Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(92)

Avis (2)

N° de cat : 15821-1-AP

Synonymes

PKM1, PK-M1, PKM1 pyruvate kinase, muscle, PKM, Pyruvate kinase PKM

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Galerie de données de validation

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  WB analysis using 15821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15821-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15821-1-AP

  rat brain tissue lysates were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2/MCF7 cells using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on hepg2 cell and MCF7 cell. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis of mouse skeletal muscle using 15821-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 15821-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15821-1-AP (PKM1-specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 15821-1-AP

  WB results of 15821-1-AP (PKM1 antibody) at dilution of 1:1000.

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  WB analysis of mouse muscle/liver tissues using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on mouse muscle tissue and mouse liver tissue. (anti-PKM2 antibody is used as a negative control for mouse muscle and liver. Anti-PKM1 antibody is used as a negative control for mouse liver. ).

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  WB analysis of mouse heart tissue using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on mouse heart tissue. (anti-PKM1 antibody is used as a negative control.).

• 

  WB analysis of 293 cell using 15821-1-AP

  WB results of PKM1,PKM2,PKLR on 293 cell. (anti-PKM1 antibody is used as a negative control).

• 

  IP experiment of mouse brain using 15821-1-AP

  IP result of anti-PKM1-specific (IP:15821-1-AP, 4ug; Detection:15821-1-AP 1:40000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15821-1-AP (PKM1-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 15821-1-AP (PKM1-specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15821-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15821-1-AP (PKM1-specific antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules 293, cellules HepG2/MCF7, multi-cellules/tissus, tissu cardiaque de souris, tissu cérébral de rat, tissu de côlon de souris, tissu de muscle squelettique de souris, tissus musculaire/hépatique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu de gliome humain, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 82 publications below
• IHC: See 12 publications below
• IF: See 5 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

15821-1-AP cible PKM1-specific dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: NM_182470
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named PKM1 and PKM2. The primary pyruvate kinase isoform before tumour development is PK-M1; however, the primary isoform from four independent tumours is PK-M2 (PMID:18337823). This antibody is specific to PKM1 isoform.

Publications

Species	Application	Title
human,mouse	WB,IHC	Nature; The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth.; Authors - Christofk Heather R HR ; KD Validated
human		Nat Immunol; NKILA lncRNA promotes tumor immune evasion by sensitizing T cells to activation-induced cell death.; Authors - Di Huang
human	WB	Sci Transl Med; ATP citrate lyase drives vascular remodeling in systemic and pulmonary vascular diseases through metabolic and epigenetic changes; Authors - Yann Grobs
human	WB,CoIP	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Nat Commun; Interactome analysis reveals that lncRNA HULC promotes aerobic glycolysis through LDHA and PKM2.; Authors - Chunqing Wang
human	WB	Nat Commun; The HIF-1α antisense long non-coding RNA drives a positive feedback loop of HIF-1α mediated transactivation and glycolysis.; Authors - Fang Zheng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Swati (Verified Customer) (09-26-2025)

The antibody functions perfectly with no background. Clear band observed at 58kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 in 5% milk in TBS-T
• Cell Tissue Type: Human lung pericytes

FH

Pallavi (Verified Customer) (03-31-2025)

I used 1:5000 anti-PKM1 15821-1-AP, it worked perfectly. The reviews were quite helpful to decide which antibody to buy. The delivery was on time. Just make sure the delivery company hands over the package to you. so better to keep a track of the package.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: purified protein

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