Anticorps Polyclonal de lapin anti-PKM

• Phare
• Validé par KD/KO

PKM Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

17

N° de cat : 10078-2-AP

Synonymes

CTHBP, OIP 3, OIP3, Opa interacting protein 3, p58, PK2, PK3, PKM, PKM1, PKM2, PKM2 pyruvate kinase, muscle, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, TCB, THBP1, Tumor M2 PK

---

Galerie de données de validation

• 

  WB analysis of HEK-293 cells using 10078-2-AP

  WB result of PKM antibody (10078-2-AP, 1:1000) with si-Control and si-PKM transfected HEK293 cells.

• 

  WB analysis using 10078-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of multi-cells/tissue using 10078-2-AP

  WB result of 10078-2-AP (PKM antibody) with various lysates.

• 

  WB analysis of rat skeletal muscle using 10078-2-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10078-2-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10078-2-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10078-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10078-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10078-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 10078-2-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 10078-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 10078-2-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10078-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10078-2-AP (PKM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10078-2-AP (PKM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 10078-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10078-2-AP (PKM antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 10078-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PKM antibody (10078-2-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HepG2 using 10078-2-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human PKM (10078-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules Raji, cellules SH-SY5Y, cellules SKOV-3, multi-cellules/tissus, tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de rat, tissu musculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

10078-2-AP cible PKM dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKM Protéine recombinante Ag0110
• Nom complet: PKM pyruvate kinase, muscle
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 58 kDa
• Numéro d’acquisition GenBank: BC000481
• Symbole du gène: PKM
• Identification du gène (NCBI): 5315
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKM, also named as OIP3, PK2, PK3, PKM1/2, p58, THBP1, CTHBP and Tumor M2-PK, belongs to the pyruvate kinase family. It is glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation. PKM plays a general role in caspase independent cell death of tumor cells. PKM has 2 isoforms named as PKM1/M2. The activity of the M2 isoform can be inhibited by tyrosine kinase signalling in tumourcells. The primary pyruvate kinase isoform before tumour development is PKM1; however, the primary isoform from four independent tumours is PKM2.(PMID:18337823). This antibody can recognize both PKM1 and PKM2.

Publications

Species	Application	Title
mouse	WB	Sci Adv; CGG repeat RNA G-quadruplexes interact with FMRpolyG to cause neuronal dysfunction in fragile X-related tremor/ataxia syndrome.; Authors - Sefan Asamitsu
pig	WB	Br J Pharmacol; PMID: 30515768; Authors - Xiaoying Lian
human	WB	Oncotarget; CDC5L drives FAH expression to promote metabolic reprogramming in melanoma.; Authors - Zhichao Gu
pig	WB	Sci Rep; Proteomic analysis indicates that mitochondrial energy metabolism in skeletal muscle tissue is negatively correlated with feed efficiency in pigs.; Authors - Liangliang Fu
human	IF	Int J Biol Sci; Dissection of Targeting Molecular Mechanisms of Aristolochic Acid-induced Nephrotoxicity via a Combined Deconvolution Strategy of Chemoproteomics and Metabolomics.; Authors - Qian Zhang
mouse	WB	Am J Physiol Endocrinol Metab; PMID: 31211620; Authors - Yang Yang